Changes in soil microbial community and activity in warm temperate forests invaded by moso bamboo (Phyllostachys pubescens)

Published Date
October 2016, Volume 21, Issue 5, pp 235–243

Original Article

First Online: 

25 June 2016

DOI: 10.1007/s10310-016-0533-6

Cite this article as: 

Wang, X., Sasaki, A., Toda, M. et al. J For Res (2016) 21: 235. doi:10.1007/s10310-016-0533-6

Author

• Xin WangEmail author
• Akiko Sasaki
• Motomu Toda
• Takayuki Nakatsubo

Abstract

the past few decades, moso bamboo (Phyllostachys pubescens) forests in Japan have rapidly expanded, and moso bamboo is now invading nearby native forests. In this study, we assessed the effects of moso bamboo invasion on the soil microbial community and activity in warm temperate forests in western Japan. We sampled soil, measured soil microbial respiration, and used phospholipid fatty acid (PLFA) analysis to examine changes in microbial community composition. We found that the invasion of bamboo into the native secondary forest of Japan can cause changes to some soil properties. We also observed a significant difference in soil microbial community composition between the bamboo and native forests. The ratio of bacterial PLFA to fungal PLFA was significantly higher after bamboo invasion, while bacterial PLFA contents were significantly lower in the organic layer. Soil microbial respiration rates significantly decreased in the organic layer, and significantly increased in the mineral layer. Microbial respiration activity, as indicated by soil microbial respiration rates per total PLFA content, decreased in the organic layer but increased in the mineral layer after bamboo invasion. These results indicate that bamboo invasion significantly affects associated soil microbial communities and decomposition patterns of soil organic matter.

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Establishment of a high-frequency regeneration system in Cerasus humilis, an important economic shrub

Published Date
October 2016, Volume 21, Issue 5, pp 244–250

Original Article

First Online: 

19 July 2016

DOI: 10.1007/s10310-016-0535-4

Cite this article as: 

Wang, R.F., Huang, F.L., Zhang, J. et al. J For Res (2016) 21: 244. doi:10.1007/s10310-016-0535-4

Author

• Rui Fang Wang
• Feng Lan Huang
• Jian Zhang
• Qiu Yan Zhang
• Li Na Sun
• Xing Shun SongEmail autho

Abstract

Cerasus humilis is a species of small, perennial, drought-resistant and multipurpose deciduous shrub grown in arid and semi-arid conditions in northern China. In this study, an efficient protocol for the rapid micropropagation of C. humilis has been standardized using stem and/or leaf explants. Direct multiple shoot induction was observed when the stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The highest shoot induction was obtained when stem explants from adult trees were cultured on MS medium supplemented with 2.0 mg L−1 6-benzyladenine (6-BA) and 0.9 mg L−1 α-naphthaleneacetic acid (NAA). The leaf and stem explants cultured on MS medium with 1.0 mg L−1 6-BA and 0.6 mg L−1 NAA, and 0.5 mg L−1 6-BA and 0.8 mg L−1NAA, respectively, produced the highest induction frequency of callus. Maximum proliferation of callus was observed on MS medium containing a combination of 0.5 mg L−16-BA with 0.6 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-d). Optimal shoots differentiated from callus were obtained on MS medium supplemented with 5.0mg L−1 6-BA and 0.9 mg L−1 NAA. In vitro rooting was achieved on half-strength (1/2) MS medium containing 0.5 mg L−1 NAA. Rooted plantlets were hardened under control conditions and successfully acclimatized under field conditions.

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