Optimization of media constituents for the production of lipase in solid state fermentation by Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)

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DOI: 10.4236/abb.2010.12016

Author(s)    

Sarat Babu Imandi, Sita Kumari Karanam, Hanumantha Rao Garapati

ABSTRACT

The production of extra cellular lipase in Solid State Fermentation (SSF) using Yarrowia lipolytica NCIM 3589 with Palm Kernal cake (Elaeis guineensis) has been studied. Different parameters such as incubation time, inoculum level, initial moisture content, carbon level and nitrogen level of the medium were optimized. Screening of various process variables has been accomplished with the help of Plackett-Burman design. The maximum lipase activity of 18.58 units per gram of dry fermented substrate (U/gds) was observed with the substrate of Palm Kernal cake in four days of fermentation.

References

[1]	Sharma, R., Chisti, Y. and Banerjee, U.C. (2001) Production, purification, characterization, and applications of lipases. Biotechnology Advances, 19(8), 627-662.
[2]	Jaeger, K.E., Steinbuchel, A. and Jendrossek, D. (1995) Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: Bacterial lipases hydrolyze poly(-hydroxyalkanoates). Applied Environmental and Microbiology, 61(8), 3113-3118.
[3]	Mochizuki, M., Hirano, M., Kanmuri, Y., Kudo, K. and Tokiwa, Y. (1995) Hydrolysis of polycaprolactone by lipase: effects of draw ratio on enzymatic degradation. Journal of Applied Polymer Science, 55(2), 289-296.
[4]	Ashok, P. (2003) Solid state fermentation. Biochemical Engineering Journal, 13(2-3), 81-84.
[5]	Castilho, L.R., Polato, C.M.S., Baruque, E.A., Sant Anna Jr, G.L. and Freire, D.M.G. (2000) Economic analysis of lipase production by Penicillium restrictum in solid-state and submerged fermentations. Biochemical Engineering Journal, 4(33), 239-247.
[6]	Gombert, A.K., Pinto, A.L., Castilho, L.R. and Freire, D.M.G. (1999) Lipase production by Penicillium restrictum in solid-state fermentation using babassu oil cake as substrate. Process Biochemistry, 35(1-2), 85-90.
[7]	Cordova, J., Nemmaoui, M., Ismaili-Alaoui, M., Morin, A., Roussos, S., Raimbault, M. and Benjilali, B. (1998) Lipase production by solid state fermentation of olive cake and sugar cane bagasse. Journal of Molecular Catalysis B: Enzymatic, 5(1-4), 75-78.
[8]	Kamini, N.R., Mala, J.G.S. and Puvanakrishnan, R. (1998) Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake. Process Biochemistry, 33(5), 505-511.
[9]	Mahadik, N.D., Puntambekar, U.S., Bastawde, K.B., Khire, J.M. and Gokhale, D.V. (2002) Production of acidic lipase by Aspergillus niger in solid state fermentation. Process Biochemistry, 38(5), 715-721.
[10]	Rao, P.V., Jayaraman, K. and Lakshmanan, C.M. (1993) Production of lipase by Candida rugosa in solid state fermentation. 1: Determination of significant process variables. Process Biochemistry, 28(6), 385-389.
[11]	Mahanta, N., Gupta, A. and Khare, S.K. (2008) Production of protease and lipase by solvent tolerant Pseudomonas aeruginosa PseA in solid-state fermentation using Jatropha curcas seed cake as substrate. Bioresource Technology, 99(6), 1729-1735.
[12]	PORLA (2000) Palm Oil Statistics. Ministry of Primary Industries, Kuala Lumpur.
[13]	Diaz, M.J.C., Rodríguez, J.A., Roussos, S., Cordova, J., Abousalham, A., Carriere, F. and Baratti, J. (2006) Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures. Enzyme and Microbial Technology, 39(5), 1042-1050.
[14]	Raimbault, M. (1998) General and microbiological aspects of solid substrate fermentation. Electronic Journal of Biotechnology, 1(3), 174 189.
[15]	Salunkhe, D.K., Chavan, J.K., Adsule, R.N. and Kadam, S.S. (1999) World oil seeds chemistry, Technology, and Utilization. Van Nostrand Reinhold, NewYork.
[16]	Imandi, S.B., Bandaru, V.V.R., Somalanka, S.R., Bandaru, S.R. and Garapati, H.R. (2008) Application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste. Bioresource Technology, 99(10), 4445-4450.
[17]	Imandi, S.B. (2008) Studies on the production of lipase in solid state fermentation using Artificial Neural Networks and Genetic Algorithms. Ph.D. Thesis, Andhra University, Visakhapatnam, India.
[18]	Winkler, U.K. and Stuckmann, M. (1979) Glycogen, hyaluronate and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. Journal of Bacteriology, 138(3), 663-670.
[19]	Plackett, R.L. and Burman, J.P. (1946) The Design of Optimum Multifactorial Experiments. Biometrika, 33(4), 305-325.
[20]	Shirazi, S.H., Rahman, S.R. and Rahman, M.M. (1998) Production of Extra cellular lipases by Saccharomyces cerevisiae. World Journal of Microbiology and Biotechnology, 14(4), 595-597.
[21]	Ul-Haq, I., Idrees, S. and Rajoka, M.I. (2002) Production of lipases by Rhizopus oligosporus by solid-state fermentation. Process Biochemistry, 37(6), 637-641.
[22]	Benjamin, S. and Pandey, A. (1997) Coconut cake: a potent substrate for production of lipase by Candida rugosa in solid state fermentation. Acta Biotechnologica, 17(3), 241-251.
[23]	Mudgetti, R.E. (1986) Manual of industrial biotechnology. American Society for Microbiology, Washington DC.
[24]	Babu, I.S. and Rao, G.H. (2007) Lipase production by Yarrowia lipolytica NCIM 3589 in solid state fermentation using mixed substrate. Research Journal of Microbiology, 2(5), 469-474.
[25]	Silman, R.W., Conway, M.F., Anderson, R.A. and Bagley, E.B. (1979) Production of aflatoxin in corn by large scale solid state fermentation process. Biotechnology and Bioengineering, 21(10), 1799-1808.
[26]	Pérez-Guerra, N., Torrado-Agrasar, A., López-Macias, C. and Pastrana, L. (2003) Main characteristics and applications of solid substrate fermentation. Electronic Journal of Environmental, Agricultural and Food Chemistry, 2(3), 343-350.

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