Blog List

Monday 17 July 2017

Synthesis and characterization of anti-bacterial and anti-fungal citrate-based mussel-inspired bioadhesives

Published Date
Received 16 December 2015, Revised 27 January 2016, Accepted 31 January 2016, Available online 2 February 2016.

Author
JinshanGuoa. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace1. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.WeiWanga. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceb. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace1. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.JianqingHua. Numbers and letters correspond to the affiliation list. Click to expose these in author workspacec. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace1. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.DenghuiXied. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.EthanGerharda. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.MerisaNisica. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.DingyingShana. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.GuoyingQianb. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.SiyangZhenga. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace. Author links open the author workspace.JianYanga. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceOpens the author workspaceOpens the author workspace
a
Department of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA
b
Zhejiang Provincial Top Key Discipline of Bioengineering, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
c
School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China
d
Department of Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Academy of Orthopedics of Guangdong Province, Guangzhou 510630, China

https://doi.org/10.1016/j.biomaterials.2016.01.069

Abstract

Bacterial and fungal infections in the use of surgical devices and medical implants remain a major concern. Traditional bioadhesives fail to incorporate anti-microbial properties, necessitating additional anti-microbial drug injection. Herein, by the introduction of the clinically used and inexpensive anti-fungal agent, 10-undecylenic acid (UA), into our recently developed injectable citrate-based mussel-inspired bioadhesives (iCMBAs), a new family of anti-bacterial and anti-fungal iCMBAs (AbAf iCs) was developed. AbAf iCs not only showed strong wet tissue adhesion strength, but also exhibited excellent in vitro cyto-compatibility, fast degradation, and strong initial and considerable long-term anti-bacterial and anti-fungal ability. For the first time, the biocompatibility and anti-microbial ability of sodium metaperiodate (PI), an oxidant used as a cross-linking initiator in the AbAf iCs system, was also thoroughly investigated. Our results suggest that the PI-based bioadhesives showed better anti-microbial properties compared to the unstable silver-based bioadhesive materials. In conclusion, AbAf iCs family can serve as excellent anti-bacterial and anti-fungal bioadhesive candidates for tissue/wound closure, wound dressing, and bone regeneration, especially when bacterial or fungal infections are a major concern.
For further details log on website :
http://www.sciencedirect.com/science/article/pii/S0142961216000958

Activating relaxation-controlled diffusion mechanisms for tailored moisture resistance of gelatin-based bioadhesives for engineered wood products

Published Date
Received 20 November 2015, Revised 9 February 2016, Accepted 11 February 2016, Available online 27 February 2016

https://doi.org/10.1016/j.compositesa.2016.02.013

Author
  • Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, ECOT 441 UCB 428, Boulder, CO 80309-0428, USA

Abstract


The feasibility of tailoring the moisture resistance of bioadhesives by activating relaxation-controlled diffusion mechanisms is demonstrated herein using gelatin, a hydrophilic biopolymer, as a model biobased resin for engineered wood products. The effect of gelatin-to-water concentration and tannin addition on the governing kinetics of water transport in gelatin-based bioadhesives was investigated in this work. Time-dependent flexural mechanical properties of laminated (a) gelatin and (b) gelatin–tannin wood veneer composites conditioned at both moderate and high humidity were characterized and compared to oriented strand board and plywood. Results indicate that increases in both gelatin and tannin content not only decrease rates of water uptake, volumetric swelling, and maximum moisture contents of gelatin-based resins, but also increasingly induce relaxation-controlled moisture diffusion behavior, which implies short-term moisture resistance and long-term moisture affinity. This behavior could be leveraged to address both in-service (i.e., strength, stiffness) and out-of-service (i.e., rapid biodegradation) requirements for engineered wood products.

For further details log on website :
http://www.sciencedirect.com/science/article/pii/S1359835X16000762

Identification of manganese-toxicity-responsive genes in roots of two citrus species differing in manganese tolerance using cDNA-AFLP

Published Date
Volume 31, Issue 3pp 813–831

Author
  • Chen-Ping Zhou
  • Chun-Ping Li
  • Wei-Wei Liang
  • Peng Guo
  • Lin-Tong Yang
  • Li-Song Chen
  1. 1.
  2. 2.
  3. 3.
Original Article
Abstract

Key message

We identified more Mn-toxicity-responsive genes from Mn-intolerantCitrus grandisthan from Mn-tolerantCitrus sinensisroots. These findings increased our understanding of the molecular mechanisms on plant Mn toxicity and Mn tolerance.

Abstract

Manganese (Mn) toxicity is the most important factor limiting crop production after aluminum toxicity in acidic soils. However, little is known about Mn-toxicity-induced alterations of gene expression profiles in woody plants. Using cDNA-AFLP, we identified 87 and 63 Mn-toxicity-responsive genes from Mn-intolerant ‘Sour pummelo’ (Citrus grandis) and Mn-tolerant ‘Xuegan’ (Citrus sinensis) roots. Among these genes, only 22 genes with the same accession number were shared by both. Protein phosphorylation/dephosphorylation-related genes were upregulated in C. sinensis roots, and downregulated in C. grandis roots except for one differentially expressed gene. Sulfur metabolism-related genes were repressed only in Mn-toxic C. grandis roots. Obviously, great differences existed in Mn-toxicity-induced alterations of gene expression profiles between C. sinensis and C. grandis roots. Genes related to protein phosphorylation/dephosphorylation (i.e., cyclin-dependent kinase-activating kinase assembly factor-related protein and PP2A regulatory subunit TAP46), cellular transport (i.e., Ca-transporting ATPase 1), and nucleic acid (i.e., ethylene-responsive transcription factor ERF109-like, structural maintenance of chromosomes protein 4-like, RNA-binding protein and DEAD-box ATP-dependent RNA helicase 21), cell wall (i.e., pectin methylesterase 1 and invertase/pectin methylesterase inhibitor family protein) and fatty acid (i.e., carboxylesterase 20) metabolisms might play a role in C. sinensis Mn tolerance. In addition, cell wall materials were increased in Mn-toxic C. sinensis and C. grandis roots, especially in the former. Interestingly, lignin content was increased in Mn-toxic C. sinensis roots, while a reverse trend was displayed in Mn-toxic C. grandis roots. In conclusion, our results provided novel clues to the molecular mechanisms on Mn toxicity and Mn tolerance in higher plants.

Keywords

cDNA-AFLP Cell wall Citrus grandis Citrus sinensis Manganese toxicity Root gene expression 

Supplementary material

468_2016_1507_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 23 kb)
For further details log on website :
https://link.springer.com/article/10.1007%2Fs00468-016-1507-1

Advantages and Disadvantages of Fasting for Runners

Author BY   ANDREA CESPEDES  Food is fuel, especially for serious runners who need a lot of energy. It may seem counterintuiti...