Proteomic changes during tuber dormancy release process revealed by iTRAQ quantitative proteomics in potato

Published Date

January 2015, Vol.86:181–190, doi:10.1016/j.plaphy.2014.12.003

Research article

Title 

Proteomic changes during tuber dormancy release process revealed by iTRAQ quantitative proteomics in potato

• Author 

• Bailin Liu ^a,b
• Ning Zhang ^a,c
• Shuo Zhao ^d
• Jing Chang ^a,c
• Zemin Wang ^a,c
• Guodong Zhang ^a,c
• Huaijun Si ^a,c,,
• Di Wang ^a,b,,

• aGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China
• bCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China
• cCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China
• dFaculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, NB E3B 6C2, Canada

Received 19 September 2014. Accepted 3 December 2014. Available online 4 December 2014.

Highlights

• •
  The first iTRAQ quantitative proteomics on tuber dormancy breaking was conducted.
• •
  The differentially expressed proteins and genes were compared.
• •
  The mechanism of tuber dormancy release was discussed with other plant models.

Abstract

Given that limited information is available with regard to tuber dormancy release related proteome, we conducted proteome analysis of tuber dormancy release process at dormant tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) using the iTRAQ technology. A total of 1,752 proteins were identified. Among them, a subset of 316 proteins was screened as significant up- (137) and down regulated (179) between DT vs DRT. A subset of 120 proteins experienced significant up- (40) or down-regulation (80) between DRT vs ST. The differentially expressed proteins were grouped into 11 functional categories. Proteins enriched in functional categories of major carbohydrate (CHO) metabolism, glycolysis, fermentation, amino acid metabolism, protein and transport were highly up-regulated, while functional categories of photosynthesis and RNA were down-regulated between DT vs DRT. Proteins enriched in functional groups of protein, cell wall, lipid metabolism, miscellaneous, and signaling were strongly up-regulated, while functional categories of photosynthesis, hormone metabolism and protein were down-regulated between DRT vs ST. Consistent with previous documented differentially expressed genes, most of differentially expressed proteins were also identified between DT and DRT, indicating the metabolism shift from growth suspension to growth activation as tubers dormancy breaking. The changes in protein profiles showed lower concordance with corresponding alterations in transcript levels, indicating possible transcriptional and posttranscriptional regulation. Furthermore, the possible mechanism of tuber dormancy release was discussed in relation to what was known in transcripts change and other plant models from carbohydrate metabolism, protein metabolism, stress response, redox regulation, transcription regulation, DNA metabolism, amino acid metabolism, development, signaling as well as hormone metabolism.

Keywords

• Dormancy
• iTRAQ
• Potato tuber
• Proteome
• Sprouting

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• ∗ 
  Corresponding author. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
• ∗∗ 
  Corresponding author. Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.

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http://www.sciencedirect.com/science/article/pii/S0981942814003660