Transcriptional profiling by DDRT-PCR analysis reveals gene expression during seed development in Carya cathayensis Sarg.

Published Date

June 2015, Vol.91:28–35, doi:10.1016/j.plaphy.2015.03.008

Research article

Title 

Transcriptional profiling by DDRT-PCR analysis reveals gene expression during seed development in Carya cathayensis Sarg.

• Author 

• You-Jun Huang ^a,b,1,
• Qin Zhou ^c,1,
• Jian-Qin Huang ^a,b,
• Yan-Ru Zeng ^a,b,
• Zheng-Jia Wang ^a,b,
• Qi-Xiang Zhang ^a,b,
• Yi-Hang Zhu ^a,
• Chen Shen ^a,
• Bing-Song Zheng ^a,b,,

• aNurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an, Hangzhou 311300, PR China
• bCenter for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Lin'an, Hangzhou 311300, PR China
• cJinhua Academy of Agricultural Sciences, Jinhua 321017, PR China

Received 28 July 2014. Accepted 24 March 2015. Available online 25 March 2015.

Highlights

• •
  Batches of unique genes involved in seed development were obtained by DDRT PCR.
• •
  Seed development in hickory underwent complex metabolisms, besides lipid synthesis.
• •
  Six genes related to lipid synthesis in seed development were further discussed.

Abstract

Hickory (Carya cathayensis Sarg.) seed has one of the highest oil content and is rich in polyunsaturated fatty acids (PUFAs), which kernel is helpful to human health, particularly to human brain function. A better elucidation of lipid accumulation mechanism would help to improve hickory production and seed quality. DDRT-PCR analysis was used to examine gene expression in hickory at thirteen time points during seed development process. A total of 67 unique genes involved in seed development were obtained, and those expression patterns were further confirmed by semi-quantitative RT-PCR and real time RT-PCR analysis. Of them, the genes with known functions were involved in signal transduction, amino acid metabolism, nuclear metabolism, fatty acid metabolism, protein metabolism, carbon metabolism, secondary metabolism, oxidation of fatty acids and stress response, suggesting that hickory underwent a complex metabolism process in seed development. Furthermore, 6 genes related to fatty acid synthesis were explored, and their functions in seed development process were further discussed. The data obtained here would provide the first clues for guiding further functional studies of fatty acid synthesis in hickory.

Keywords

• Hickory
• Differential mRNA display
• Fatty acid synthesis
• Gene differential expression

Abbreviations

• BAP, BCR-associated protein
• C4H, cinnamate 4-hydroxylase
• DAP, days after pollination
• DDRT-PCR, differential display reverse transcriptase polymerase chain reaction
• FA, fatty acid
• FAD, fatty acid desaturase
• FAH12, fatty acid hydroxylase 12
• GO, gene ontology
• MES9, methyl esterase 9
• MUFA, monounsaturated fatty acid
• NAD1, NADH dehydrogenase subunit 1
• PUFAs, polyunsaturated fatty acids
• SAD, stearoyl-ACP desturases
• SUCLG, succinyl-CoA ligase
• TDFs, transcript-derived fragments
• TUB, tubulin
• TFs, transcription factors
• TAG, triacylglycerol
• VLPUFAs, very long polyunsaturated fatty acids

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• ∗ 
  Corresponding author. Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an, Hangzhou 311300, PR China.

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