Water-compatible molecularly imprinted polymers for selective solid phase extraction of dencichine from the aqueous extract of Panax notoginseng

Published Date

1 January 2016, Vol.1008:225–233, doi:10.1016/j.jchromb.2015.11.053

Title 

Water-compatible molecularly imprinted polymers for selective solid phase extraction of dencichine from the aqueous extract of Panax notoginseng

• Author 

• Wenhua Ji ^a
• Hongkai Xie ^b
• Jie Zhou ^a,c
• Xiao Wang ^a,b,,
• Xiuli Ma ^b
• Luqi Huang ^c,,

• aKey Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China
• bCollege of Food Science and Engineering, Shandong Agricultural University, Taian 270018, China
• cNational Resource Center for Chinese Materia Medica, State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing 100700, China

Received 15 September 2015. Revised 27 November 2015. Accepted 29 November 2015. Available online 2 December 2015.

Highlights

• •
  Multi-templates molecularly imprinted polymers for dencichine were firstly proposed.
• •
  The MIPs displayed excellent hydrophilicity.
• •
  The MIPs had high affinity and selectivity to dencichine.
• •
  Application of the optimized protocol for the extraction of dencichine from the aqueous extract of Panax notoginseng.

Abstract

Specific molecularly imprinted polymers for dencichine were developed for the first time in this study by the bulk polymerization using phenylpyruvic acid and dl-tyrosine as multi-templates. The photographs confirmed that molecularly imprinted polymers prepared using N,N′-methylene diacrylamide as cross-linker and glycol dimethyl ether as porogen displayed excellent hydrophilicity. Selectivity, adsorption isotherm and adsorption kinetics were investigated. The sample loading-washing-eluting solvent was optimized to evaluate the property of molecularly imprinted solid phase extract. Compared with LC/WCX-SPE, water-compatible molecularly imprinted solid phase extraction displayed more excellent specific adsorption performance. The extracted dencichine from Panax notoginseng with the purity of 98.5% and the average recovery of 85.6% (n = 3) was obtained.

Keywords

• Molecularly imprinted polymers
• HPLC
• Natural products
• Dencichine
• Panax notoginseng

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• DOCX (759K)

• ⁎ 
  Corresponding author at: Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan 250014, China. Fax: +86 531 82964889.
• ⁎⁎ 
  Corresponding author. Fax: +86 10 84027175.

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