Published Date
January 2015, Volume 70, Issue 1, pp 149–187

Bioluminescent fungi from Peninsular Malaysia—a taxonomic and phylogenetic overview

• Author 

• Audrey L. C. Chew, 
• Dennis E. Desjardin, 
• Yee-Shin Tan, 
• Md Yusoff Musa, 
• Vikineswary Sabaratnam

Abstract

Fifteen bioluminescent fungi recently collected from Peninsular Malaysia are analysed herein. The phylogeny of the Malaysian bioluminescent fungi and closely related taxa were evaluated with molecular data from the nuclear ribosomal large subunit (LSU), RNA polymerase 2 second largest subunit (RPB2) and internal transcribed spacers (ITS) gene regions. DNA sequences data support the circumscription of species based on the morphological species concept, but was unable to fully support the current sectional delimitation of genus Mycena. Of the 15 taxa analysed here, four are novel species described herein, viz. Mycena gombakensis, M. nocticaelum, M. coralliformis and Panellus luxfilamentus; while four taxa of Mycena sect. Calodontes were recently described as new. One species (M. noctilucens) represents a new distribution record to Peninsular Malaysia, and six species (Neonothopanus nambi, Filoboletus manipularis, P. luminescens, Roridomyces pruinosoviscidus, M. chlorophos and M. illuminans) have been previously reported. Roridomyces pruinosoviscidus is accepted as a new combination based on morphological and phylogenetic data. Culture morphology data indicate its potential for taxon delimitation as axenic cultures of each species produced unique and distinguishable characteristics. Comprehensive descriptions, illustrations and photographs are provided on basidiome and culture morphology. A key to aid in species identification, comparisons with allied species and data on basidiome and mycelium luminescence are also provided. This study gives the first report on mycelial luminescence for M. noctilucens, P. luminescens, R. pruinosoviscidus as well as all new species described herein. The reports in this study bring the total known luminescent fungi worldwide to 81 species.

Keywords

Basidiomycete Biodiversity Culture morphology Filoboletus Mycena NeonothopanusPanellus Phylogeny Roridomyces Systematics Taxonomy

Electronic supplementary material

The online version of this article (doi:10.1007/s13225-014-0302-9) contains supplementary material, which is available to authorized users.

References

1. Airth RL, Foerster GE (1960) Some aspects of fungal bioluminescence. J Cell Comp Physiol 56:173–182PubMedCrossRef
2. Aravindakshan DM, Kumar TKA, Manimohan P (2012) A new bioluminescent species of Mycena sect. Exornatae from Kerala State, India. Mycosphere 3(5):556–561CrossRef
3. Boidin J, Lanquetin P (1983) Basidiomycetes Aphyllophorales epitheloides etalés. Mycotaxon 16:461–499
4. Buchan A, Newell SY, Moreta JIL, Moran MA (2002) Analysis of internal transcribed spacer (ITS) regions of rRNA genes in fungal communities in a Southeastern U.S. Salt Marsh. Microb Ecol 43:329–340PubMedCrossRef
5. Burdsall HH, Miller OK Jr (1975) A reevaluation of Panellus and Dictyopanus (Agaricales). Beih Nova Hedwigia 51:79–91
6. Capelari M, Desjardin DE, Perry BA, Asai T, Stevani CV (2011) Neonothopanus gardneri: a new combination for a bioluminescent agaric from Brazil. Mycologia 103:1433–1440PubMedCrossRef
7. Chew ALC, Tan YS, Desjadin DE, Musa MY, Sabaratnam V (2013) Taxonomic and phylogenetic re-evaluation of Mycena illuminans. Mycologia 105:1325–1335PubMedCrossRef
8. Chew ALC, Tan YS, Desjadin DE, Musa MY, Sabaratnam V (2014) Four new bioluminescent taxa of Mycena sect. Calodontes from Peninsular Malaysia, Mycologia
9. Choi YW, Hyde KD, Ho WH (1999) Single spore isolation of fungi. Fungal Divers 3:29–38
10. Corner EJH (1950) Descriptions of two luminous tropical agarics (Dictyopanus and Mycena). Mycologia 42:423–431CrossRef
11. Corner EJH (1954) Further descriptions of luminous agarics. Trans Br Mycol Soc 37:256–271CrossRef
12. Corner EJH (1981) The Agaric Genera Lentinus, Panus, and Pleurotus. Nova Hedwigia, Germany
13. Corner EJH (1986) The agaric genus Panellus Karst. (including Dictyopanus Pat.) in Malaysia. Gard Bull Singapore 39:103–147
14. Corner EJH (1994) Agarics in Malesia. I. Tricholomatoid. II. Mycenoid. Beih Nova Hedwigia 109:165–271
15. Deheyn DD, Latz MI (2007) Bioluminescence characteristics of a tropical terrestrial fungus (basidiomycetes). Luminescence 22:462–467PubMedCrossRef
16. Desjardin DE (1990) Culture morphology of Marasmius species. Sydowia 42:17–87
17. Desjardin DE, Boonpratuang T, Hywel-Jones N (2003) New spinose species of Mycena in sections Basipedes and Polyadelphia from Thailand. Fungal Divers 12:7–17
18. Desjardin DE, Oliveira AG, Stevani CV (2008) Fungi bioluminescence revisited. Photochem Photobiol Sci 7:170–182PubMedCrossRef
19. Desjardin DE, Perry BA, Lodge DJ, Stevani CV, Nagasawa E (2010) Luminescent Mycena: new and noteworthy species. Mycologia 102:459–477PubMedCrossRef
20. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the Bootstrap. Evolution 39(4):783–791CrossRef
21. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity of basidiomycetes—application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118PubMedCrossRef
22. Harkin JM, Obst JR (1973) Syringaldazine, an effective reagent for detecting laccase and peroxidase in fungi. Experentia 39:381–387CrossRef
23. Harvey EN (1952) Bioluminescence. Academic, New York
24. Herring PJ (1994) Luminous fungi. Mycologist 8:181–183CrossRef
25. Huelsenbeck JP, Ronquist F (2005) Bayesian analysis of molecular evolution using MrBayes. In: Nielsen R (ed) Statistical methods in molecular evolution. Springer Verlag, New York, pp 183–232CrossRef
26. Kobayasi Y (1951) Contributions to the luminous fungi from Japan. J Hattori Bot Lab 5:1–6
27. Kornerup A, Wanscher JH (1963) Methuen handbook of colour. Methuen & Co Ltd., London
28. Largent D, Johnson D, Watling R (1977) How to identify mushrooms to genus III: microscopic features. Eureka Printing Co. Inc., California
29. Largent DL (1986) How to identify mushrooms to genus I: macroscopic features. Eureka Printing Co. Inc., California
30. Lee SS, Alias SA, Jones EGB, Zainuddin N, Chan HT (2012) Checklist of fungi of Malaysia. Swan Printing Sdn. Bhd, Malaysia
31. Liu KL, Porras-Alfaro A, Kuske CR, Eichorst SA, Xie G (2012) Accurate, rapid taxonomic classification of fungal large-subunit rRNA genes. Appl Environ Microbiol 78:1523–1533PubMedCentralPubMedCrossRef
32. Liu YL, Whelen S, Hall BD (1999) Phylogenetic relationships among Ascomycetes: evidence from an RNA polymerase II subunit. Mol Biol Evol 16:1799–1808PubMedCrossRef
33. Maas Geesteranus RA (1992) Mycenas of the Northern Hemisphere. II. Conspectus of the Mycenas of the Northern Hemispere. Verh Kon Ned Akad Wetensch, Afd Natuurk, Tweede Reeks 90:1–493
34. Maddison WP, Maddison DR (2011) Mesquite: a modular system for evolutionary analysis. Version 2.75. http://mesquiteproject.org
35. Marr CD (1986) The taxonomic potential of laccase and tyrosinase spot tests. Mycologia 78:169–184CrossRef
36. Matheny PB (2005) Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe, Agaricales). Mol Phylogenet Evol 35:1–20PubMedCrossRef
37. Matheny PB, Curtis JM, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Yang ZL, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis M, Daniele GM, Desjardin DE, Kropp BR, Norvell LL, Parker A, Vellinga EC, Vilgalys R, Hibbett DS (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98:982–995PubMedCrossRef
38. Matheny PB, Liu YJ, Ammirati JF, Hall BD (2002) Using RPB1 sequences to improve phylogenetic inference among mushrooms (Inocybe, Agaricales). Am J Bot 89(4):688–698PubMedCrossRef
39. Matheny PB, Wang Z, Binder M, Curtis JM, Lim YW, Nilsson RH, Hughes KW, Hofstetter V, Ammirati JF, Schoch CL, Langer E, Langer G, McLaughlin DJ, Wilson AW, Frøslev T, Ge ZW, Kerrigan RW, Slot JC, Yang ZL, Baroni TJ, Fischer M, Hosaka K, Matsuura K, Seidl MT, Vauras J, Hibbett DS (2007) Contributions of rpb2 and tef1 to phylogeny of mushrooms and allies (Basidiomycota, Fungi). Mol Phylogenet Evol 43:430–451PubMedCrossRef
40. Moncalvo J-M, Vilgalys R, Redhead SA, Johnson JE, James TY, Aime MC, Hofstetter V, Verduin SJW, Larsson E, Baroni TJ, Thorn RG, Jacobsson S, Clemencon H, Miller OK Jr (2002) One hundred and seventeen clades of euagarics. Mol Phylogenet Evol 23:357–400PubMedCrossRef
41. Nobles MK (1948) Studies in forest pathology. VI. Identification of cultures of wood-rotting fungi. Can J Res 26:281–431PubMedCrossRef
42. Nobles MK (1965) Identification of cultures of wood-inhabiting Hymenomycetes. Can J Bot 43:1097–1139CrossRef
43. Oliveira AG, Desjardin DE, Perry BA, Stevani CV (2012) Evidence that a single bioluminescent system is shared by all known bioluminescent fungal lineages. Photochem Photobiol Sci 11:848–852CrossRef
44. Pegler DN (1983) Agaric Flora of the Lesser Antilles (Kew Bulletin Additional Series IX). HMSO, London
45. Pegler DN (1986) Agaric Flora of Sri Lanka (Kew Bulletin Additional Series XII). Royal Botanic Gardens, Kew
46. Petersen RH, Krisai-Greilhuber I (1999) Type specimen studies in Pleurotus. Persoonia 17:201–219
47. Petersen RH, Hughes KW, Lickey EB, Kovalenko AE, Morozova OV, Psurtseva NV (2008) A new genus, Cruentomycena, with Mycena viscidocruenta as type species. Mycotaxon 105:119–136
48. Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14(9):817–818PubMedCrossRef
49. Rexer K-H (1994) Die Gatung Mycena s.l., Studien zu ihrer anatomie, morphologie und systematik. Dissertation, Universität Tübingen
50. Robich G (2003) Mycena d’Europa. A.M.B, Fondazione, Fondazione
51. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19(12):1572–1574PubMedCrossRef
52. Shih YS, Chen CY, Lin WW, Kao HW (2013) Mycena kentingensis, a new species of luminous mushroom in Taiwan, with reference to its culture method. Mycol Prog. doi:10.1007/s11557-013-0939-x
53. Singer R (1986) Agaricales in modern taxonomy. Koeltz Scientific Books, Germany
54. Stalpers JA (1978) Identification of wood-inhabiting fungi in pure culture. Stud Mycol 16:1–248
55. Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods), Version 4. Sinauer Associates, Sunderland
56. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRef
57. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246PubMedCentralPubMed
58. Vydryakova GA, Van DT, Shoukouhi P, Psurtseva NV, Bissett J (2012) Intergenomic and intragenomic ITS sequence heterogeneity in Neonothopanus nambi (Agaricales) from Vietnam. Mycology 3(2):88–99
59. Wassink EC (1978) Luminescence in fungi. In: Herring PJ (ed) Bioluminescence in action. Academic, London, pp 171–197
60. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, Inc., New York, pp 315–322CrossRef

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