Thursday 1 September 2016

Stoichiometry of soil extracellular enzyme activity along a climatic transect in temperate grasslands of northern China

Published Date

July 2016, Vol.98:74–84, doi:10.1016/j.soilbio.2016.04.008

Title

Stoichiometry of soil extracellular enzyme activity along a climatic transect in temperate grasslands of northern China

Author

Xiaoqian Peng

Wei Wang^,

Department of Ecology, College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of the Ministry of Education, Peking University, Beijing 100871, China

Received 19 November 2015. Revised 1 April 2016. Accepted 5 April 2016. Available online 12 April 2016.

Highlights

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Ratios of log-transformed C-, N- and P-acquiring enzymes were 1:1.2:1.4.
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Enzyme C:N and C:P ratios declined with soil depth, while enzyme N:P ratio remained stable.
•
Soil extracellular enzyme activity ratio was not in homeostasis but resource dependent on soil and microbial biomass stoichiometry.

Abstract

Ratios of specific carbon (C), nitrogen (N) and phosphorus (P) acquisition activities converged on 1:1:1 at a global scale and in tropical ecosystems. It is less clear if this pattern can be applied in temperate grasslands. The questions of whether the pattern remains stable across different soil depths and what the relative contributions are from the influence of climatic, edaphic abiotic and biotic factors on soil extracellular enzyme activity (EEA) stoichiometry remain uncertain. We measured potential activities of one C-acquiring enzyme (β-1, 4-glucosidase), two N-acquiring enzymes (β-N-acetylglucosaminidase and leucine aminopeptidase) and one organic P-acquiring enzyme (acid phosphatase) and major influential factors along a climatic transect in temperate grasslands of northern China during the growing season of 2013. We found lower enzyme C: N (0.47) and C: P (0.18) ratios and a higher enzyme N: P activity ratio (0.40) in 0–20 cm soil depth in temperate grasslands than in tropical soils (C: N, 1.83; C:P, 0.21; N: P, 0.13). The enzyme C: N and C: P ratios decreased with soil depth except for the enzyme C: N ratio in desert steppes. However, there were no significant differences in enzyme N: P ratio with soil depth. Among all the factors, soil total C, N and P contents accounted for the most variation in soil EEA and ecoenzymatic stoichiometry in 0–10 cm surface soil, which implied that soil EEA stoichiometry was largely controlled by soil nutrient stoichiometry. Moreover, edaphic factors had less influence on soil EEA stoichiometry in subsoil than in surface soil and edaphic abiotic factors had a larger effect on soil EEA stoichiometry than climatic and biotic factors. Our results suggest that soil extracellular enzyme activity ratios were not in homeostasis but resource dependent on soil and microbial biomass stoichiometry.