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http://www.nrcresearchpress.com/doi/abs/10.1139/m91-149#/toc/cjfr/46/11
Mature pollen grains of lodgepole pine (Pinuscontorta Dougl.) were incubated in solutions of sucrose, indoleacetic acid (IAA), and stock solution A containing boric acid, calcium nitrate, magnesium sulfate, and potassium nitrate. Sucrose solution did not stimulate pollen germination but did inhibit pollen tube growth. A slight promotion of tube growth resulted from an IAA solution, but no increase was obtained in germination percentage. Stock solution B (diluted one-tenth of concentration of stock solution A) was optimum for pollen germination and pollen tube growth. Two basic types of pollen tube development and pollen tube branching occurred. An abnormal development of the pollen tube, protruding through the saccus, was recorded. After 5 days of incubation in stock solution B, the generative cell divided into the stalk cell and the body cell, and 2 days later the body nucleus divided to form two male nuclei of either equal or unequal sizes.
For further details log on website :
http://www.nrcresearchpress.com/doi/abs/10.1139/m91-149#/toc/cjfr/46/11
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