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Tuesday 13 December 2016

Suppression of OsVPE3 Enhances Salt Tolerance by Attenuating Vacuole Rupture during Programmed Cell Death and Affects Stomata Development in Rice

Published Date
AccessOriginal article
First Online: 
29 November 2016
DOI: 10.1186/s12284-016-0138-x


Cite this article as: 
Lu, W., Deng, M., Guo, F. et al. Rice (2016) 9: 65. doi:10.1186/s12284-016-0138-x


Author 

  • Wenyun Lu
  • Minjuan Deng
  • Fu Guo
  • Mingqiang Wang
  • Zhanghui Zeng
  • Ning Han
  • Yinong Yang
  • Muyuan Zhu
  • Hongwu Bian
Abstract 

Background

Vacuolar processing enzymes (VPEs) are cysteine proteinases that act as crucial mediators of programmed cell death (PCD) in plants. In rice, however, the role of VPEs in abiotic stress-induced PCD remains largely unknown. In this study, we generated OsVPE3 overexpression and suppression transgenic lines to elucidate the function of this gene in rice.

Results

Survival rate and chlorophyll retention analyses showed that suppression of OsVPE3 clearly enhanced salt stress tolerance in transgenic rice compared with wild type. Furthermore, fragmentation of genomic DNA was inhibited in plants with down-regulated OsVPE3. Vital staining studies indicated that vacuole rupture occurred prior to plasma membrane collapse during salt-induced PCD. Notably, overexpression of OsVPE3 promoted vacuole rupture, whereas suppression of OsVPE3 attenuated or delayed the disintegration of vacuolar membranes. Moreover, we found that suppression of OsVPE3 caused decreased leaf width and guard cell length in rice.

Conclusions

Taken together, these results indicated that suppression of OsVPE3 enhances salt tolerance by attenuating vacuole rupture during PCD. Therefore, we concluded that OsVPE3 plays a crucial role in vacuole-mediated PCD and in stomatal development in rice.

Keywords

OsVPE3Programmed cell deathRiceSalt stressStomataVacuolar processing enzyme

Abbreviations

BCECF-AM
2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein-acetoxymethyl
BCL-2
B-cell lymphoma-2
GFP
Green fluorescent protein
MPK6
Mitogen-activated protein kinase 6
PCD
Programmed cell death
qRT-PCR
Real time quantitative polymerase chain reaction
ROS
Reactive oxygen species
SOS
Salt-overly-sensitive
VPE
Vacuolar processing enzymes




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