Aggregation and adhesion abilities to enterocyte-like HCT-116 cells of probiotic candidates Lactobacillus plantarum strains isolated from "mandai", Indonesian fermented food against enteropathogens

International Food Research Journal, Volume 23, Issue 5, 2016, pp. 2234-2240

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Aggregation and adhesion abilities to enterocyte-like HCT-116 cells of probiotic candidates Lactobacillus plantarum strains isolated from "mandai", Indonesian fermented food against enteropathogens

 Emmawati A. 1, Jenie B.S.L. * 2, Nuraida L. 2 ,3, Syah D. 2 ,3

Abstract : Ten probiotic candidates of L. plantarum strains isolated from "mandai" were evaluated for aggregation, adhesion to HCT-116 cells and competition abilities towards enteropathogens (Listeria monocytogenes ATCC 13932, enteropathogenic Escherichia coli (EPEC) K1.1 and Salmonella enterica serovar Typhimurium ATCC 14028). The results showed that four L. plantarum strains had good autoaggregation ability above 50%, but only two strains demonstrated coaggregation ability (above 25%) with all enteropathogens tested. Five strains of L. plantarum showed the ability to adhere to HCT-116 cells above 60%, with the highest adhesion (74%) was performed by MB427 after 1 h incubation at 37°C. L. plantarum strains showed stronger competitive inhibition, exclusion and displacement towards EPEC than S. Typhimurium and L. monocytogenes. L. plantarum MB427 strain showed strong inhibition abilities towards the three pathogens except in displacing of L. monocytogenes. These results suggest that L. plantarum strains isolated from "mandai" were promising as probiotic candidates acquired protection against certain enteropathogenic infection.

Keywords : Adhesion,Aggregation,Enteropathogen,Lactobacillus plantarum,Probiotic

Subject Area : Food Science

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