Published Date
Received 4 February 2016, Revised 12 September 2016, Accepted 12 September 2016, Available online 13 September 2016
Author
Highlights
Abstract
Adhesive interaction contributes toward tumor metastasis and the transmembrane glycoprotein receptor, integrin has been recognized to mediate the adhesion to extracellular matrix thus upregulating tumor metastasis. In the current study, we evaluated the anti-adhesive mechanisms of a water-soluble polysaccharide (BCP) extracted from Bupleurum chinense. BCP inhibited integrin-mediated adhesion of human melanoma A375 cells to fibronectin but had no effects on nonspecific adhesion to poly-l-lysine. BCP also reduced β1 integrin ligand affinity for GST-FNIII9-10 proteins. The adhesion-dependent formation of F-actin stress fiber and focal adhesion (FA) was also inhibited by BCP treatment. The inhibition of BCP on integrin-mediated signaling is probably through inhibiting phosphorylation of focal adhesion kinase (FAK) and paxillin. Collectively, our current findings indicated that BCP may be a potential therapy for melanoma metastasis due to its inhibitory effects on integrin function.
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http://www.sciencedirect.com/science/article/pii/S0144861716310888
Received 4 February 2016, Revised 12 September 2016, Accepted 12 September 2016, Available online 13 September 2016
Author
- a Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Life Science Research Center, Beihua University, Jilin 132013, China
- b Wood Material Science and Engineering Key Laboratory of Jilin Province, Beihua University, Jilin 132013, China
- c Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John’s, NL, Canada
- d Research Center for Clinical & Translational Medicine, 302 Hospital, Beijing 100039, China
- e School of Chemical Engineering, Northeast Dianli University, Jilin 132012, China
Highlights
- •
- BCP, water-soluble polysaccharide extracted from Bupleurum chinense, inhibited integrin-mediated adhesion of human melanoma A375 cells to fibronectin but had no effects on nonspecific adhesion to poly-l-lysine.
- •
- BCP-treatment reduced β1 integrin ligand affinity and inhibited the adhesion-dependent formation of F-actin stress fibers and focal adhesions.
- •
- The inhibition of BCP on integrin-mediated signaling is probably through its dephosphorylatory effects on focal adhesion kinase (FAK) and paxillin.
- •
- Our current findings indicated that BCP may be a potential therapy for melanoma metastasis due to its inhibitory effects on integrin signaling.
Abstract
Adhesive interaction contributes toward tumor metastasis and the transmembrane glycoprotein receptor, integrin has been recognized to mediate the adhesion to extracellular matrix thus upregulating tumor metastasis. In the current study, we evaluated the anti-adhesive mechanisms of a water-soluble polysaccharide (BCP) extracted from Bupleurum chinense. BCP inhibited integrin-mediated adhesion of human melanoma A375 cells to fibronectin but had no effects on nonspecific adhesion to poly-l-lysine. BCP also reduced β1 integrin ligand affinity for GST-FNIII9-10 proteins. The adhesion-dependent formation of F-actin stress fiber and focal adhesion (FA) was also inhibited by BCP treatment. The inhibition of BCP on integrin-mediated signaling is probably through inhibiting phosphorylation of focal adhesion kinase (FAK) and paxillin. Collectively, our current findings indicated that BCP may be a potential therapy for melanoma metastasis due to its inhibitory effects on integrin function.
For further details log on website :
http://www.sciencedirect.com/science/article/pii/S0144861716310888
Saikosaponin C is extracted from the root of Bupleurum chinense DC. It efficiently inhibited LPS-induced apoptotic cell death via inhibition of caspase-3 activation and caspase-3-mediated-FAK degradation. It exhibits anti-HBV activity. It may have the potential for therapeutic angiogenesis but is not suitable for cancer therapy. Saikosaponin C
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