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Tuesday, 27 June 2017

Study of ITO Glass Electrode Modified with Iron Oxide Nanoparticles and Nafion for Glucose Biosensor Application

Published Date
Available online 24 March 2016.

Noorhashimah Mohamad Nor. Author links open the author workspace.Zainovia Lockman. Author links open the author workspace.Khairunisak Abdul Razak. Author links open the author workspace.Opens the author workspaceOpens the author workspace
School of Materials and Mineral Resources Engineering Universiti Sains Malaysia, Nibong Tebal, Penang 14300 Malaysia

In this study, we report the fabrication of the indium tin oxide (ITO) glass electrode modified with iron oxide nanoparticles (IONPs) and nafion for glucose biosensor applications. The IONPs was synthesized using the precipitation method and functionalized with citric acid (CA) to provide hydrophilic surface and functional group for glucose oxidase (GOx) enzyme immobilization. The structural and morphological studies of CA-IONPs were characterized using X-ray diffractometer (XRD) and transmission electron microscope (TEM). The size of the IONPs measured from TEM image was ∼17 nm. The bioelectrode designated as Nafion/GOx/CA-IONPs/ITO was developed by drop casting of the CA-IONPs, GOx and nafion on the ITO glass. The Nafion/GOx/CA-IONPs/ITO bioelectrode showed good electrochemical performance for glucose detection. The functionalized CA-IONPs acted as the catalyst and help to improve the electron transfer rate between GOx and ITO electrode. In addition, thin nafion film was coated on the electrode to prevent interference and improve chemical stability. The Nafion/GOx/CA-IONPs/ITO bioelectrode showed high sensitivity of 70.1 ╬╝AmM-1cm-2 for the linear range of 1.0-8.0 mM glucose concentrations.


Iron oxide nanoparticles
glucose biosensor
citric acid


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Peer-review under responsibility of School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia.
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