Antibiofilm activity of carboxymethyl chitosan on the biofilms of non-Candida albicans Candida species

Published Date
20 September 2016, Vol.149:77–82, doi:10.1016/j.carbpol.2016.04.101

Title 
Antibiofilm activity of carboxymethyl chitosan on the biofilms of non-Candida albicans Candida species

• Author 

• Yulong Tan ^a,,
• Matthias Leonhard ^a,
• Doris Moser ^b,
• Berit Schneider-Stickler ^a,

• aDepartment of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria
• bDepartment of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria

Received 25 December 2015. Revised 17 April 2016. Accepted 22 April 2016. Available online 26 April 2016.

Highlights

• •
  Carboxymethyl chitosan inhibited biofilm of non-albicans Candida species.
• •
  Carboxymethyl chitosan inhibited the activity of planktonic cells grown and adhesion.
• •
  Carboxymethyl chitosan blocked further development of biofilms at different stages.

Abstract

Although most cases of candidiasis have been attributed to Candida albicans, non-C. albicans Candida species have been isolated in increasing numbers in patients. In this study, we determined the inhibition of carboxymethyl chitosan (CM-chitosan) on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata. Biofilm by all tested species in microtiter plates were inhibited nearly 70%. CM-chitosan inhibited mixed species biofilm in microtiter plates and also on medical materials surfaces. To investigate the mechanism, the effect of CM-chitosan on cell viability and biofilm growth was employed. CM-chitosan inhibited Candida planktonic growth as well as adhesion. Further biofilm formation was inhibited with CM-chitosan added at 90 min, 12 h or 24 h after biofilm initiation. CM-chitosan was not only able to inhibit the metabolic activity of Candida cells, but was also active upon the establishment and the development of biofilms.

Keywords

• Biofilm
• Non-Candida albicans Candida species
• Carboxymethyl chitosan

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• ⁎ 
  Corresponding author.

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