Comparative proteomic analysis of seed embryo proteins associated with seed storability in rice (Oryza sativa L) during natural aging

Published Date
June 2016, Vol.103:31–44, doi:10.1016/j.plaphy.2016.02.026

Research article

Comparative proteomic analysis of seed embryo proteins associated with seed storability in rice (Oryza sativa L) during natural aging

Author 

Jiadong Gao ^a,b,1

Hua Fu ^c,1

Xinqiao Zhou ^c

Zhongjian Chen ^a

Yi Luo ^a

Baiyuan Cui ^a

Guanghui Chen ^b,,

Jun Liu ^a,,

• aAgro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China
• bHunan Agricultural University, Changsha, 410128, China
• cRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

Received 25 August 2015. Revised 1 February 2016. Accepted 17 February 2016. Available online 24 February 2016.

Highlights

• •
  Rice seeds sensitive to storage display more differentially accumulated proteins.
• •
  The level of malondialdehyde increases more drastically in seeds sensitive to storage.
• •
  Redox regulation proteins (GSH related protein) may be related to seed storability.
• •
  Some disease/defense proteins play an important role in seed storability.

Abstract

Seed storability is considered an important trait in rice breeding; however, the underlying regulating mechanisms remain largely unknown. Here, we carried out a physiological and proteomic study to identify proteins possibly related to seed storability under natural conditions. Two hybrid cultivars, IIYou998 (IIY998) and BoYou998 (BY998), were analyzed in parallel because they share the same restorer line but have significant differences in seed storability. After a 2-year storage period, the germination percentage of IIY998 was significantly lower than that of BY998, whereas the level of malondialdehyde was reversed, indicating that IIY998 seeds may suffer from more severe damage than BY998 during storage. However, we did not find correlation between activities of antioxidant enzymes of superoxide dismutase, peroxidase, and catalase and seed storability. We identified 78 embryo proteins in embryo whose abundance varied more than 3-fold different during storage or between IIY998 and BY998. More proteins changed in abundance in IIY998 embryo (67 proteins) during storage than in BY998 (10 proteins). Several redox regulation proteins, mainly glutathione-related proteins, exhibited different degree of change during storage between BY998 and IIY998 and might play an important role protecting embryo proteins from oxidation. In addition, some disease/defense proteins, including DNA-damage-repair/toleration proteins, and a putative late embryogenesis abundant protein were significantly downregulated in IIY998, whereas their levels did not change in BY998, indicating that they might be correlated with seed storability. Further studies on these candidate seed storage proteins might help improve our understanding of seed aging.

Keywords

• Rice (Oryza sativa L)
• Proteomics
• Seed storability
• Natural aging
• Glutathione-related proteins
• Seed storage proteins

Abbreviations

• CAT, catalase
• GLO, glyoxalase
• GRX, glutaredoxin
• GSH, glutathione
• HSP, heat shock protein
• LEA protein, late embryogenesis abundant protein
• LOX, lipoxygenase
• MDA, malondialdehyde
• POD, peroxidase
• QTL, quantitative trait loci
• ROS, reactive oxygen species
• SOD, superoxide dismutase
• TIM, mitochondrial import inner membrane translocase

---

Fig. 1.

Fig. 2.

Fig. 3.

 Table 1

Table 1.

Fig. 4.

Fig. 5.

Fig. 6.

• DOC (32K)

Table S1

• PDF (71K)

Table S2

Fig. S1–S4.

• ∗ 
  Corresponding author. Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Jinying Rd. 20, Tianhe District, Guangzhou, 510640, China.
• ∗∗ 
  Corresponding author.

For further details log on website :

http://www.sciencedirect.com/science/article/pii/S0981942816300523