Hydrogen peroxide sensing and cytotoxicity activity of Acacia lignin stabilized silver nanoparticles

Published Date
January 2016, Vol.82:39–47, doi:10.1016/j.ijbiomac.2015.09.072

Title 
Hydrogen peroxide sensing and cytotoxicity activity of Acacia lignin stabilized silver nanoparticles

• Author 

• Keshaw Ram Aadil ^a
• Anand Barapatre ^a
• Avtar Singh Meena ^b
• Harit Jha ^a,,

• aDepartment of Biotechnology, Guru Ghasidas Vishwavidyalaya (Central University), Bilaspur 495009, Chhattisgarh, India
• bTumor Biology Laboratory, National Institute of Pathology, New Delhi 110029, India

Received 26 February 2015. Revised 13 June 2015. Accepted 29 September 2015. Available online 3 October 2015.

Highlights

. Study reported the detection of H₂O₂ using Acacia lignin mediated silver nanoparticles.
. One step method for the synthesis of AGNPs was developed.
. Sensing properties of AGNPs toward hydrogen peroxide were investigated.
. Lignin mediated AGNPs demonstrated toxicity effects in MCF-7 and A375 cell lines.

Abstract

The study is aimed at detection of hydrogen peroxide (H₂O₂) using Acacia lignin mediated silver nanoparticles (AGNPs). The synthesis of AGNPs was achieved at conditions optimized as, 3 ml of 0.02% lignin and 1 mM silver nitrate incubated for 30 min at 80 °C and pH 9. Initial screening of AGNPs was performed by measuring the surface plasmon resonance peak at 410–430 nm using UV–vis spectrophotometer. Transmission electron microscopy, atomic force microscopy, X-ray diffraction and particle size analysis confirmed the spherical shaped face centered cubic structure and 10–50 nm size of AGNPs. The infrared spectroscopy study further revealed that the active functional groups present in lignin were responsible for the reduction of silver ions (Ag⁺) to metallic silver (Ag⁰). Lignin stabilized silver nanoparticles showed good sensitivity and a linear response over wide concentrations of H₂O₂ (10⁻¹ to 10⁻⁶M). Further, the in vitrocytotoxicity activity of the lignin mediated AGNPs (5–500 μg/ml) demonstrated toxicity effects in MCF-7 and A375 cell lines. Thus, lignin stabilized silver nanoparticles based optical sensor for H₂O₂ could be potentially applied in the determination of reactive oxygen species and toxic chemicals which further expands the importance of lignin stabilized silver nanoparticles.

Graphical abstract

Keywords

• Acacia lignin
• Silver nanoparticles
• Hydrogen peroxide sensing
• In vitro cytotoxicity

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  Corresponding author.

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