Analysis of the mating system, reproductive characteristics, and spatial genetic structure in a natural mangrove tree (Bruguiera gymnorrhiza) population at its northern biogeographic limit in the southern Japanese archipelago

Published Date

April 2015, Volume 20, Issue 2, pp 293-300

First online: 30 November 2014

Title 

Analysis of the mating system, reproductive characteristics, and spatial genetic structure in a natural mangrove tree (Bruguiera gymnorrhiza) population at its northern biogeographic limit in the southern Japanese archipelago

• Author 

• Md Sajedul Islam 
• , Chunlan Lian
• , Norikazu Kameyama
• , Taizo Hogetsu

Abstract

We examined the mating system, reproductive characteristics, and spatial genetic structure in a population of Bruguiera gymnorrhiza located at its northern biogeographic limit on the Iriomote Island of the southern Japanese archipelago. Nuclear and chloroplast simple sequence repeat (nSSR and cpSSR) markers were used to analyze adult trees, seedlings and propagule samples in the Urauchi river basin. Low genetic diversity was detected at the nSSR and a predominant haplotype was identified at the cpSSR. The mean multilocus outcrossing rate (0.855) significantly differed from 1.0, suggesting a relatively higher selfing rate. Substantial inbreeding was suggested from biparental inbreeding rates and significant F IS within the adult and seedling cohorts. A wide coverage of pollen and propagule dispersal was inferred within the study population based on a paternity analysis of the propagules and a parentage analysis of the seedlings; moreover, a lack of spatial genetic structure was observed. Our results suggest that B. gymnorrhiza in the Urauchi river basin was most likely established from a few founders and formed a homogeneous population due to limited gene flow from southern or central populations.

Keywords

Bruguiera gymnorrhiza Genetic diversity Mangrove Mating system and reproductive characteristics SSR or microsatellite marker

References

1. Chybicki IJ, Burczyk J (2009) Simultaneous estimation of null alleles and inbreeding coefficients. J Hered 100:106–113CrossRefPubMed
2. Dow BD, Ashley MV (1996) Microsatellite analysis of seed dispersal and parentage of saplings in bur oak, Quercus macrocarpa. Mol Ecol 5:615–627CrossRef
3. Franceschinelli EV, Bawa KS (2000) The effect of ecological factors on the mating system of a South American shrub species (Helicteres brevispira). Heredity 84:116–123CrossRefPubMed
4. Frankham R (1998) Inbreeding and extinction: island populations. Conserv Biol 12:665–675CrossRef
5. Ge JP, Cai BY, Ping WX, Song G, Ling HZ, Lin P (2005) Mating system and population genetic structure of Bruguiera gymnorrhiza (Rhizophoraceae), viviparous mangrove species in China. J Exp Mar Biol Ecol 326:48–55CrossRef
6. Geng Q, Lian C, Goto S, Tao J, Kimura M, Islam MS, Hogetsu T (2008) Mating system, pollen and propagule dispersal, and spatial genetic structure in a high-density population of the mangrove tree Kandelia candel. Mol Ecol 17:4724–4739CrossRefPubMed
7. Giang LH, Geada GL, Hong PN, Tuan MS, Lien NTH, Ikeda S, Harada K (2006) Genetic variation of two mangrove species in Kandelia (Rhizophoraceae) in Vietnam and surrounding area revealed by microsatellite markers. Int J Plant Sci 167:291–298CrossRef
8. Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486
9. Hardy OJ, Vekemans X (2002) SPAGeDi: a versatile computer program to analyze spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620CrossRef
10. Islam MS, Lian C, Kameyama N, Wu B, Hogetsu T (2004) Development of microsatellite markers in Rhizophora stylosa using a dual-suppression-polymerase chain reaction technique. Mol Ecol Notes 4:110–112CrossRef
11. Islam MS, Lian CL, Kameyama N, Wu B, Hogetsu T (2006a) Development and characterization of ten new microsatellite markers in a mangrove tree species Bruguiera gymnorrhiza (L.). Mol Ecol Notes 6:30–32CrossRef
12. Islam MS, Tao JM, Geng QF, Lian CL, Hogetsu T (2006b) Isolation and characterization of eight compound microsatellite markers in a mangrove tree Kandelia candel (L.) Druce. Mol Ecol Notes 6:1111–1113CrossRef
13. Islam MS, Lian CL, Geng QF, Kameyama N, Hogetsu T (2008) Chloroplast microsatellite markers for the mangrove tree species Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa, and cross-amplification in other mangrove species. Conserv Genet 9:989–993CrossRef
14. Islam MS, Lian C, Kameyama N, Hogetsu T (2012) Analyses of genetic population structure of two ecologically important mangrove tree species, Bruguiera gymnorrhiza and Kandelia obovata from different river basins of Iriomote Island of the Ryukyu Archipelago, Japan. Tree Genet Genomes 8:1247–1260CrossRef
15. Islam MS, Lian C, Kameyama N, Hogetsu T (2014) Low genetic diversity and limited gene flow in a dominant mangrove tree species (Rhizophora stylosa) at its northern biogeographical limit across the chain of three Sakishima islands of the Japanese archipelago as revealed by chloroplast and nuclear SSR analysis. Plant Syst Evol 300:1123–1136
16. Kondo K, Nakamura T, Tsuruda K (1987) Pollination in Bruguiera gymnorrhiza and Rhizophora mucronata in Ishigaki Island, the Ryukyu islands, Japan. Brotropica 19:377–380CrossRef
17. Loiselle BA, Sork VL, Nason J, Graham C (1995) Spatial genetic structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae). Am J Bot 82:1420–1425CrossRef
18. Maguire TL, Saenger P, Baverstock P, Henry R (2000) Microsatellite analysis of genetic structure in the mangrove species Avicennia marina (Forsk.) Vierh. (Avicenniaceae). MolEcol 9:1853–1862
19. Marshall TC, Slate J, Kruuk L, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655CrossRefPubMed
20. Minobe S, Fukui S, Saiki R, Kajita T, Changtragoon S, Shukor NA, Latiff A, Ramesh BR, Koizumi O, Yamazaki T (2010) Highly differentiated population structure of a Mangrove species, Bruguiera gymnorrhiza (Rhizophoraceae) revealed by one nuclear GapCp and one chloroplast intergenic spacer trnF–trnL. Conserv Genet 11:301–310CrossRef
21. Nakasuga T (1979) Analysis of mangrove stand. Sci Bull Coll Agric Univ Ryukyus 26:413–500 (in Japanese with English summary)
22. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
23. Ritland K (2002) Extensions of models for the estimation of mating systems using n independent loci. Heredity 88:221–228CrossRefPubMed
24. Sandoval-Castro E, Mu˜niz-Salazara R, Enríquez-Paredesb LM, Riosmena-Rodríguezc R, Dodd RS, Tovilla-Hernándeze C, Arredondo-García MC (2012) Genetic population structure of red mangrove (Rhizophora mangle L.) along the northwestern coast of Mexico. Aquat Bot 99:20–26CrossRef
25. Takayama K, Tamura M, Tateishi Y, Kajita T (2009) Isolation and characterization of microsatellite loci in a mangrove species, Rhizophora stylosa (Rhizophoraceae). Conserv Genet Resour 1:175–178CrossRef
26. Takeuchi T, Sugaya T, Kanazashi A, Yoshimaru H, Katsuta M (2001) Genetic diversity of Kandelia candel and Bruguiera gymnorrhiza in the Southwest Islands, Japan. J For Res 6:157–162CrossRef
27. Tomlinson PB (1986) The Botany of mangroves. Cambridge University Press, Cambridge
28. Tomlinson PB, Bunt JS (1979) Preliminary observations on floral biology in mangrove Rhizophoraceae. Biotropica 11:256–277CrossRef

For further details log on website :
http://link.springer.com/article/10.1007/s10310-014-0473-y