Author
Abstract
The selenium supply in almost all European countries is below the recommended daily intake, and different strategies are followed to fortify foods. In the present work, the influence of germination of garden cress (Lepidium sativum cv. Ogrodowa) in different selenium solutions (Na2SeO3 and Na2SeO4) on Se uptake, total antioxidant capacity, glucosinolates, protein, and amino acids was studied. Cytotoxicity in HL-60 human leukemic cell line was also assessed. The addition of selenite (Na2SeO3) or selenate (Na2SeO4) led to a significant increment in Se uptake in garden cress sprouts, and the highest Se content was observed at 8 mg/L in both inorganic Se solutions (36−38 μg/g of dm). The Se-enriched sprouts presented a large total antioxidant capacity (142−157 μmol of Trolox/g of dm), total glucosinolate content (99−124 μg/g of dm), protein (36−37% dm), and total essential amino acid content (40−41 g/100 g of protein), and no cytotoxicity on HL-60 human leukemic cells was observed. Garden cress sprouts obtained with selenite solution at 8 mg/L presented the best nutritional qualities and might provide a substantial proportion of Se in European diets. Bearing in mind the high nutritional value of sprouts, these may serve for the production of functional foods.
For further details log on website :
http://pubs.acs.org/doi/abs/10.1021/jf903458u
† Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain
‡ Department of Animal Nutrition and Feed Management, University of Technology and Life Sciences Bydgoszcz, str. Mazowiecka 28, 85-084, Bydgoszcz, Poland
§ Institute of Animal Reproduction and Food Research (PAN), Tuwima 10, 10-747 Olsztyn, Poland
# Institute of Bioorganic Chemistry (PAN), Z. Noskowskiego 12/14, 61-704 Poznan, Poland
J. Agric. Food Chem., 2010, 58 (4), pp 2331–2336
DOI: 10.1021/jf903458u
Publication Date (Web): January 27, 2010
Copyright © 2010 American Chemical Society
*Corresponding author (telephone + 34 915622900, ext. 241; fax + 34 915644873; e-mail ificv12@ifi.csic.es).
Abstract
The selenium supply in almost all European countries is below the recommended daily intake, and different strategies are followed to fortify foods. In the present work, the influence of germination of garden cress (Lepidium sativum cv. Ogrodowa) in different selenium solutions (Na2SeO3 and Na2SeO4) on Se uptake, total antioxidant capacity, glucosinolates, protein, and amino acids was studied. Cytotoxicity in HL-60 human leukemic cell line was also assessed. The addition of selenite (Na2SeO3) or selenate (Na2SeO4) led to a significant increment in Se uptake in garden cress sprouts, and the highest Se content was observed at 8 mg/L in both inorganic Se solutions (36−38 μg/g of dm). The Se-enriched sprouts presented a large total antioxidant capacity (142−157 μmol of Trolox/g of dm), total glucosinolate content (99−124 μg/g of dm), protein (36−37% dm), and total essential amino acid content (40−41 g/100 g of protein), and no cytotoxicity on HL-60 human leukemic cells was observed. Garden cress sprouts obtained with selenite solution at 8 mg/L presented the best nutritional qualities and might provide a substantial proportion of Se in European diets. Bearing in mind the high nutritional value of sprouts, these may serve for the production of functional foods.
For further details log on website :
http://pubs.acs.org/doi/abs/10.1021/jf903458u
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