Root Glucosinolate Profiles for Screening of Radish (Raphanus sativus L.) Genetic Resources

Author

Gibum Yi*†‡, Sooyeon Lim†§, Won Byoung Chae∥, Jeong Eun Park†, Hye Rang Park†, Eun Jin Lee†§, and Jin Hoe Huh*†‡§

†Department of Plant Science, ‡Plant Genomics and Breeding Institute, §Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea

∥ Department of Horticultural Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Korea

J. Agric. Food Chem., 2016, 64 (1), pp 61–70

DOI: 10.1021/acs.jafc.5b04575

Publication Date (Web): December 17, 2015

Copyright © 2015 American Chemical Society

*Tel: +82-2-880-4562; fax: +82-2-873-2056; e-mail: gibumyi@gmail.com (G.Y.)., *Tel: +82-2-880-4562; fax: +82-2-873-2056; e-mail: huhjh@snu.ac.kr (J.H.H.).

Abstract

Radish (Raphanus sativus L.), a root vegetable, is rich in glucosinolates (GLs), which are beneficial secondary metabolites for human health. To investigate the genetic variations in GL content in radish roots and the relationship with other root phenotypes, we analyzed 71 accessions from 23 different countries for GLs using HPLC. The most abundant GL in radish roots was glucoraphasatin, a GL with four-carbon aliphatic side chain. The content of glucoraphasatin represented at least 84.5% of the total GL content. Indolyl GL represented only 3.1% of the total GL at its maximum. The principal component analysis of GL profiles with various root phenotypes showed that four different genotypes exist in the 71 accessions. Although no strong correlation with GL content and root phenotype was observed, the varied GL content levels demonstrate the genetic diversity of GL content, and the amount that GLs could be potentially improved by breeding in radishes.

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