Blog List

Thursday, 17 November 2016

Changes in soil microbial community and activity in warm temperate forests invaded by moso bamboo (Phyllostachys pubescens)

Published Date
Volume 21, Issue 5, pp 235–243

Original Article
DOI: 10.1007/s10310-016-0533-6

Cite this article as: 
Wang, X., Sasaki, A., Toda, M. et al. J For Res (2016) 21: 235. doi:10.1007/s10310-016-0533-6

  • Xin Wang
  • Akiko Sasaki
  • Motomu Toda
  • Takayuki Nakatsubo

the past few decades, moso bamboo (Phyllostachys pubescens) forests in Japan have rapidly expanded, and moso bamboo is now invading nearby native forests. In this study, we assessed the effects of moso bamboo invasion on the soil microbial community and activity in warm temperate forests in western Japan. We sampled soil, measured soil microbial respiration, and used phospholipid fatty acid (PLFA) analysis to examine changes in microbial community composition. We found that the invasion of bamboo into the native secondary forest of Japan can cause changes to some soil properties. We also observed a significant difference in soil microbial community composition between the bamboo and native forests. The ratio of bacterial PLFA to fungal PLFA was significantly higher after bamboo invasion, while bacterial PLFA contents were significantly lower in the organic layer. Soil microbial respiration rates significantly decreased in the organic layer, and significantly increased in the mineral layer. Microbial respiration activity, as indicated by soil microbial respiration rates per total PLFA content, decreased in the organic layer but increased in the mineral layer after bamboo invasion. These results indicate that bamboo invasion significantly affects associated soil microbial communities and decomposition patterns of soil organic matter.


  1. Arao T, Okano S, Nishio T (2001) Comparison of bacterial and fungal biomass determined by phospholipid fatty acid and direct microscopical analysis in 4 types of upland soils. Soil Microorg 55:29–36Google Scholar
  2. Bardgett RD, Freeman C, Ostle NJ (2008) Microbial contributions to climate change through carbon cycle feedbacks. ISME J 2:2805–2814CrossRefGoogle Scholar
  3. Batten KM, Scow KM, Davies KF, Harrison SP (2006) Two invasive plants alter soil microbial community composition in serpentine grasslands. Biol Invasion 8:217–230CrossRefGoogle Scholar
  4. Bekku Y, Koizumi H, Oikawa T, Iwaki H (1997) Examination of four methods for measuring soil respiration. Appl Soil Ecol 5(3):247–254CrossRefGoogle Scholar
  5. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefPubMedGoogle Scholar
  6. Chang EH, Chiu CY (2015) Changes in soil microbial community structure and activity in a cedar plantation invaded by moso bamboo. Appl Soil Ecol 91:1–7CrossRefGoogle Scholar
  7. Chornesky EA, Bartuska AM, Aplet GH, Britton KO, Cummings-Carlson J, Davis FW, Eskow J, Gordon DR, Gottschalk KW, Haack RA, Hansen AJ, Mack RN, Rahel FJ, Shannon MA, Wainger LA, Wigley TB (2005) Science priorities for reducing the threat of invasive species to sustainable forestry. Bioscience 55:335–348CrossRefGoogle Scholar
  8. Fontaine S, Barot S, Barré P, Bdioui N, Mary B, Rumpel C (2007) Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature 450:277–280CrossRefPubMedGoogle Scholar
  9. Frostegård A, Bååth E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fertil Soils 22:59–65CrossRefGoogle Scholar
  10. Frostegård A, Bååth E, Tunlid A (1993) Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biol Biochem 25:723–730CrossRefGoogle Scholar
  11. Fukushima K, Usui N, Ogawa R, Tokuchi N (2015) Impacts of moso bamboo (Phyllostachys pubescens) invasion on dry matter and carbon and nitrogen stocks in a broad-leaved secondary forest located in Kyoto, western Japan. Plant Species Biol 30:81–95CrossRefGoogle Scholar
  12. Isagi Y, Torii A (1998) Range expansion and its mechanisms in a naturalized bamboo species, Phyllostachys pubescens, in Japan. J Sustain For 6:127–141CrossRefGoogle Scholar
  13. Ishiga H, Dozen K, Kodera Y, Haito K (2001) Effects of bamboo invasion on the soil of broadleaf forests and their potential environmental impact. Geosci Rep Shimane Univ 20:83–86 (in Japanese with English summary)Google Scholar
  14. Keane RM, Crawley MJ (2002) Exotic plant invasions and the enemy release hypothesis. Trends Ecol Evol 17:164–170CrossRefGoogle Scholar
  15. Kobayashi T, Tada M (2010) How do moso bamboo forests change carbon sequestration and storage, and decomposition of soil organic matter in community forests? Shinrin Kagaku 53:6–10 (in Japanese)Google Scholar
  16. Kourtev PS, Ehrenfelda JG, Häggblom M (2002) Exotic plant species alter the microbial community structure and function in the soil. Ecology 83:3152–3166CrossRefGoogle Scholar
  17. Kourtev PS, Ehrenfelda JG, Häggblom M (2003) Experimental analysis of the effect of exotic and native plant species on the structure and function of soil microbial communities. Soil Biol Biochem 35:895–905CrossRefGoogle Scholar
  18. Li WH, Zhang CB, Jiang HB, Xin GR, Yang ZY (2006) Changes in soil microbial community associated with invasion of the exotic weed, Mikania micrantha H.B.K. Plant Soil 281:309–324CrossRefGoogle Scholar
  19. Myers RT, Zak DR, White DC, Peacock A (2001) Landscape-level patterns of microbial community composition and substrate use in upland forest ecosystems. Soil Sci Soc Am J 65:359–367CrossRefGoogle Scholar
  20. Ohtonen R, Fritze H, Pennanen T (1999) Ecosystem properties and microbial community changes in primary succession on a glacier forefront. Oecologia 119:239–246CrossRefGoogle Scholar
  21. R Core Team (2015) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Accessed May 2015
  22. Shibata S (2010) Bamboo forest management for efficient use of bamboo materials. Shinrin Kagaku 53:15–19 (in Japanese)Google Scholar
  23. Shinohara Y, Kyoichi O (2015) Comparisons of soil water content between a moso bamboo (Phyllostachys pubescens) forest and an evergreen broadleaved forest in western Japan. Plant Species Biol 30:96–103CrossRefGoogle Scholar
  24. Stoffel W, Chu F, Ahrens EH (1959) Analysis of long-chain fatty acids by gas–liquid chromatography. Micromethod for preparation of methyl esters. Anal Chem 31:307–308CrossRefGoogle Scholar
  25. Tang X, Fan S, Qi L, Guan F, Cai C, Du M (2015) Soil respiration and carbon balance in a moso bamboo (Phyllostachys heterocycla (Carr.) Mitford cv. Pubescens) forest in subtropical China. iForest 8:606–614
  26. Torii A (2003) Bamboo forests as invaders of surrounding secondary forests. J Jpn Soc Reveg Technol 28:412–416 (in Japanese)CrossRefGoogle Scholar
  27. Ueda K (1960) Studies on the physiology of bamboo, with reference to practical application. Resource bureau ref. data 34. Resource Bureau Science and Technics Agency, Prime Minister’s Office, Tokyo
  28. Wang X, Ren H (2008) Comparative study of the photo-discoloration of moso bamboo (Phyllostachys pubescens Mazel) and two wood species. Appl Surf Sci 254:7029–7034CrossRefGoogle Scholar
  29. White DC, Davis WM, Nickels JS, King JD, Bobbie RJ (1979) Determination of the sedimentary microbial biomass by extractable lipid phosphate. Oecologia 40:51–62CrossRefGoogle Scholar
  30. Wilcove DS, Rothstein D, Dubow J, Phillips A, Losos E (1998) Quantifying threats to imperiled species in the United States. BioScience 48(8):607–615CrossRefGoogle Scholar
  31. Wolfe BE, Klironomos JN (2005) Breaking new ground: soil communities and exotic plant invasion. Bioscience 55:477–487CrossRefGoogle Scholar
  32. Xu QF, Jiang PK, Wu JS, Zhou GM, Shen RF, Fuhrmann JJ (2015) Bamboo invasion of native broadleaf forest modified soil microbial communities and diversity. Biol Invasions 17:433–444CrossRefGoogle Scholar
  33. Xue D, Yao HY, Ge DY, Huang CY (2008) Soil microbial community structure in diverse land use systems: a comparative study using Biolog, DGGE, and PLFA analyses. Pedosphere 18:653–663CrossRefGoogle Scholar
  34. Yoshitake S, Nakatsubo T (2008) Changes in soil microbial biomass and community composition along vegetation zonation in a coastal sand dune. Aust J Soil Res 47:390–396CrossRefGoogle Scholar
  35. Yoshitake S, Uchida M, Nakatsubo T, Kanda H (2006) Characterization of soil microflora on a successional glacier foreland in the High Arctic on Ellesmere Island, Nunavut, Canada using phospholipid fatty acid analysis. Polar Biosci 19:73–84Google Scholar
  36. Zhang N, Liu W, Yang H, Yu X, Gutknecht JLM, Zhang Z, Wan S, Ma K (2013) Soil microbial responses to warming and increased precipitation and their implications for ecosystem C cycling. Oecologia 173:1125–1142CrossRefPubMedGoogle Scholar

For further details log on website :

No comments:

Post a Comment

Mangrove Forest Management & Restoration

The Sabah Forestry Department has conserved most if not all Mangrove Forests under Class V for marine life conservation and as a natural me...