Wednesday, 23 November 2016

Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture

Published Date
Volume 27, Issue 6pp 1257–1263

Original Paper
DOI: 10.1007/s11676-016-0217-2

Cite this article as: 
Wang, S., Li, C., Wang, H. et al. J. For. Res. (2016) 27: 1257. doi:10.1007/s11676-016-0217-2

Author
  • Shujie Wang
  • Chun Li
  • Hujun Wang
  • Xiangmei Zhong
  • Jing Zhao
  • Yueying Tong
  • Yajun Zhou
Abstract
In this study, using Taxus cuspidata as a raw material, we obtained stable high-yielding cell lines by subculturing and quantified paclitaxel content using ultrasonic extraction combined with TLC–UV spectrophotometry. In single factor and multiple factors tests to optimize design and study the effects of elicitors, precursors, and metabolic inhibitors on paclitaxel production by Taxus cuspidata cells, paclitaxel production reached 4.32 mg/L when 100 μmol/L methyl jasmonate, 20 mg/L salicylic acid, 400 mg/L phenylalanine and 2 mg/L gibberellin (GA3) were added to the culture medium of suspension cells. When adding metabolic adjustment factors on the 7th day of culture, extra- and intracellular paclitaxel production was the highest at 4.855 mg/L, paclitaxel release rate was 10.48 %, fresh mass and paclitaxel production of cell increased, respectively, by 6.08 and 11.57 %. By controlling the anabolism of paclitaxel, paclitaxel yield was significantly improved.

Keywords

ElicitorPrecursorMetabolic inhibitorPaclitaxelCell cultureTaxus cuspidata

For further details log on website :
http://link.springer.com/article/10.1007/s11676-014-0479-5

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