Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture

Published Date
December 2016, Volume 27, Issue 6, pp 1257–1263

Original Paper

First Online: 

03 June 2016

DOI: 10.1007/s11676-016-0217-2

Cite this article as: 

Wang, S., Li, C., Wang, H. et al. J. For. Res. (2016) 27: 1257. doi:10.1007/s11676-016-0217-2

Author

• Shujie Wang
• Chun Li
• Hujun Wang
• Xiangmei Zhong
• Jing Zhao
• Yueying Tong
• Yajun Zhou

Abstract

In this study, using Taxus cuspidata as a raw material, we obtained stable high-yielding cell lines by subculturing and quantified paclitaxel content using ultrasonic extraction combined with TLC–UV spectrophotometry. In single factor and multiple factors tests to optimize design and study the effects of elicitors, precursors, and metabolic inhibitors on paclitaxel production by Taxus cuspidata cells, paclitaxel production reached 4.32 mg/L when 100 μmol/L methyl jasmonate, 20 mg/L salicylic acid, 400 mg/L phenylalanine and 2 mg/L gibberellin (GA3) were added to the culture medium of suspension cells. When adding metabolic adjustment factors on the 7th day of culture, extra- and intracellular paclitaxel production was the highest at 4.855 mg/L, paclitaxel release rate was 10.48 %, fresh mass and paclitaxel production of cell increased, respectively, by 6.08 and 11.57 %. By controlling the anabolism of paclitaxel, paclitaxel yield was significantly improved.

Keywords

ElicitorPrecursorMetabolic inhibitorPaclitaxelCell cultureTaxus cuspidata

Project funding: This work was supported by development plan project during “the 12th Five Year Plan” Nation Science and Technology in rural area (No. 2012AA10A506-04 and No. 2013AA103005-04), Changchun City science and technology development program (No. 2014174) and Changchun City science and technology support program (No. 2014NK002).

The online version is available at http://www.springerlink.com

Corresponding editor: Chai Ruihai

References 

1. Gao MB, Li XT, Ruan CJ (2011) The callus induction of Taxus cuspidata. J Dali Natl Univ 13(3):256–259Google Scholar
2. He DY, Yang F, Shi L, Liu TX, Zhang ZS (2010) Effects of hormones on taxol Taxus cuspidata nana accumulation in callus. J Southwest Agric Univ 23(2):538–541Google Scholar
3. Kan CR, Cui JM, Wang JW, Wang WZ, Zhao YQ (2009) Determination of content of taxol in Taxus cuspidata extracting paclitaxel after residue. Chin Mod Tradit Med Compos 11(2):27–28Google Scholar
4. Kwon IC, Yoo YJ, Lee JH, Hyun JO (1998) Enhancement of taxol production by in situ recovery of product. Process Biochem 33(7):701–707CrossRefGoogle Scholar
5. Laskaris G, Bounkhay M, Theodoridis G, Vander Heijden R, Verpoorte R, Jaziri M (1999) Induction of geranyl–geranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate. Plant Sci 147:1–8CrossRefGoogle Scholar
6. Li H (2009) The technology of extraction of taxol from Taxus cuspidata leaves. Chin Pharm 18(19):44–45Google Scholar
7. Li LQ, Fu CH, Yu LJ, Wang S, Yu LJ (2009) Effects of synchronization making taxol content in Taxus cells synergistic induction. Plant Physiol Commun 45(3):253–257Google Scholar
8. Liu BY, Ye HC (1999) The research of biological production of taxol. Biotechnol Bull 34(7):5–7Google Scholar
9. Mei XG, Zhou ZQ, Wu QJ (2001) The influence of elicitor, precursor and inhibitors on taxol biosynthesis. Biotechnology 5:11Google Scholar
10. Qiao LJ, Man RL, Ni WD, Liang YH (2009) Research on extraction and purification of taxol in the branches of Taxus media. Chin J Chin Med 34(8):973–976Google Scholar
11. Raskin I (1992) Salicylate, a new plant hormone. Plant Physiol 99:799–803CrossRefPubMedPubMedCentralGoogle Scholar
12. Seki M, Ohzora C, Takeda M, Furusaki S (1997) Taxol (Paclitaxel) production using free and immobilized cells of Taxus cuspidata. Biotechnol Bioeng 53:214–219CrossRefPubMedGoogle Scholar
13. Tang ZH, Zhong WD (2006) Molecular mechanism of paclitaxel antitumor. Chin Clin Rehabil 10(27):125–127Google Scholar
14. Yu XH, Shao JH, Yuan ZH, Zhao CH, Dai YQ (2013) The progress of plant cell engineering technology research on production of taxol. J Northwest 33(6):1279–1284Google Scholar
15. Zhai HH, Fu YL (2010) The Taxus tissue culture. Dev For Sci Technol 24(4):98–100Google Scholar
16. Zhai XX, Yang J, Li YY (2009) A new method making yew high yield cell line establishment and update fast. Biol Technol Commun 20(3):376–379Google Scholar
17. Zhang XR (2009) Study on optimizing of environmental conditions of Taxus cuspidata cell culture to produce taxol. Master’s degree thesis, Jilin university, Jilin, pp 15–35
18. Zhao JP, Yang SS (2014) Establishment of cell suspension culture of Taxus media. J Northwest Agric For Univ (Nat Sci Ed) 42(1):189–195Google Scholar
19. Zhong JJ (2002) Plant cell culture for production of paclitaxel and other taxanes. J Biosci Bioeng 94:591–599CrossRefPubMedGoogle Scholar
20. Zhou ZQ, Mei XG, Wu QJ (2002) Regulation of taxol production in cell suspension cultures of taxus chinensis by elicitors, precursors and inhibitors. Nat Prod Res Dev 14(2):19–21Google Scholar

For further details log on website :
http://link.springer.com/article/10.1007/s11676-014-0479-5