Published Date
Available online 27 May 2016, doi:10.1016/j.ijbiomac.2016.05.095
alginate
membranes
Poloxamer 188
cytotoxicity
wound dressings
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http://www.sciencedirect.com/science/article/pii/S0141813016305098
Available online 27 May 2016, doi:10.1016/j.ijbiomac.2016.05.095
In Press, Accepted Manuscript — Note to users
Title
COMPARISON OF THE PROPERTIES OF MEMBRANES PRODUCED WITH ALGINATE AND CHITOSAN FROM MUSHROOM AND FROM SHRIMP
Received 6 April 2016. Revised 14 May 2016. Accepted 26 May 2016. Available online 27 May 2016.
Highlights
. Dense and porous chitosan-alginate (CA) membranes were produced for coatings of skin wounds.
. The influence of chitosan source (fungal or animal) on properties and toxicity was evaluated.
. Porosity increased in membranes obtained with fungal chitosan and with its molecular mass.
. The indirect cytotoxicity to fibroblasts cells was low for membranes with fungal chitosan.
. The replacement of animal chitosan by fungal chitosan to produce CA membranes is promising
Abstract
Dense and porous chitosan-alginate membranes (1:1 in mass) useful as coverages of skin wounds treated through cell therapy were produced using chitosan of different chain sizes from fungal (white mushrooms) and animal (shrimp shells) sources. Porous materials were obtained by adding the surfactant Poloxamer 188 to the formulations. The influence of chitosan type on membranes physicochemical properties and toxicity to fibroblasts was evaluated. Porosity was noticed to be more pronounced in membranes obtained with fungal chitosan and increased with its molecular mass. These formulations showed the highest values of thickness, roughness, opacity, liquid uptake and water vapor permeability. The membranes were not toxic to fibroblasts, but the lowest values (0.16-0.21%) were observed for membranes prepared with fungal chitosan in the presence of surfactant. In conclusion, it is possible to replace chitosan from animal sources by chitosan of fungal origin to produce membranes with negligible cytotoxicity while maintaining appropriate physicochemical properties.
Keywords
fungal chitosan
Highlights
. Dense and porous chitosan-alginate (CA) membranes were produced for coatings of skin wounds.
. The influence of chitosan source (fungal or animal) on properties and toxicity was evaluated.
. Porosity increased in membranes obtained with fungal chitosan and with its molecular mass.
. The indirect cytotoxicity to fibroblasts cells was low for membranes with fungal chitosan.
. The replacement of animal chitosan by fungal chitosan to produce CA membranes is promising
Abstract
Dense and porous chitosan-alginate membranes (1:1 in mass) useful as coverages of skin wounds treated through cell therapy were produced using chitosan of different chain sizes from fungal (white mushrooms) and animal (shrimp shells) sources. Porous materials were obtained by adding the surfactant Poloxamer 188 to the formulations. The influence of chitosan type on membranes physicochemical properties and toxicity to fibroblasts was evaluated. Porosity was noticed to be more pronounced in membranes obtained with fungal chitosan and increased with its molecular mass. These formulations showed the highest values of thickness, roughness, opacity, liquid uptake and water vapor permeability. The membranes were not toxic to fibroblasts, but the lowest values (0.16-0.21%) were observed for membranes prepared with fungal chitosan in the presence of surfactant. In conclusion, it is possible to replace chitosan from animal sources by chitosan of fungal origin to produce membranes with negligible cytotoxicity while maintaining appropriate physicochemical properties.
Keywords
For further details log on website :
http://www.sciencedirect.com/science/article/pii/S0141813016305098