Prebiotic potential of pectins and pectic oligosaccharides derived from lemon peel wastes and sugar beet pulp: A comparative evaluation

Published Date

January 2016, Vol.20:108–121, doi:10.1016/j.jff.2015.10.029

Title 

Prebiotic potential of pectins and pectic oligosaccharides derived from lemon peel wastes and sugar beet pulp: A comparative evaluation

• Author 

• Belén Gómez ^a,b
• Beatriz Gullón ^c
• Remedios Yáñez ^a,b
• Henk Schols ^d
• José L. Alonso ^a,b,,

• aDepartment of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain
• bCITI-Tecnopole, San Ciprián de Viñas, 32901 Ourense, Spain
• cCenter for Biotechnology and Fine Chemistry (CBQF), Portuguese Catholic University, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal
• dLaboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands

Received 31 March 2015. Revised 16 October 2015. Accepted 20 October 2015. Available online 10 November 2015.

Highlights

• •
  Refined POS and pectins from lemon peels and sugar beet were evaluated for their prebiotic potential using faecal inocula.
• •
  These substrates showed different DP, DA, DM and neutral sugars to galacturonic acid ratios.
• •
  The populations of eight groups of bacteria belonging to the gut microbiota were measured by FISH.
• •
  SBPOS showed a strong bifidogenic potential whereas LPOS allowed a selective increase of lactobacilli.
• •
  The profile of organic acids generated was dependent on the substrate considered.

Abstract

Sugar beet pulp (SBP) and lemon peel wastes (LPW) were used to obtain two mixtures of pectic oligosaccharides (denoted as SBPOS and LPOS, respectively). Oligogalacturonides in LPOS showed a larger molecular weight, higher degree of methylation and lower degree of acetylation than the ones in SBPOS. The suitability of pectic oligosaccharides, pectins from SBP and LPW and commercial FOS for causing prebiotic effects were compared by in vitro fermentation and fluorescence in situhybridization using human faecal inocula and eight different probes. The joint populations of bifidobacteria and lactobacilli increased from 19% up to 29%, 34% and 32% in cultures with LPOS, SBPOS and FOS, respectively. Faecalibacterium and Roseburia also increased their counts with all the substrates (especially with LPOS). The highest concentrations of organic acids were observed in media containing oligosaccharides. This work confirms that pectic oligosaccharides present better prebiotic properties than pectins, and similar or better than FOS.

Keywords

• Sugar beet pulp
• Lemon peel wastes
• Prebiotics
• Pectic oligosaccharides
• Pectins
• FISH

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• *
  Corresponding author. Department of Chemical Engineering, Faculty of Science, University of Vigo (Campus Ourense), As Lagoas, 32004 Ourense, Spain. Tel.: +34 988 38 70 47; fax: +34 988 38 70 01.
  
  

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