In vitro evaluation of the structural and bioaccessibility of kenaf seed oil nanoemulsions stabilised by binary emulsifiers and β-cyclodextrin complexes

Published Date

November 2016, Vol.189:90–98, doi:10.1016/j.jfoodeng.2016.06.002

Title 

In vitro evaluation of the structural and bioaccessibility of kenaf seed oil nanoemulsions stabilised by binary emulsifiers and β-cyclodextrin complexes

• Author 

• Ai Mun Cheong ^a
• Chin Ping Tan ^b
• Kar Lin Nyam ^a,,

• aDepartment of Food Science with Nutrition, Faculty of Applied Sciences, UCSI University, 56000, Kuala Lumpur, Malaysia
• bDepartment of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Received 6 April 2016. Revised 1 June 2016. Accepted 4 June 2016. Available online 7 June 2016.

Highlights

• •
  A two-stage in vitro model was employed.
• •
  To investigate the release behaviour of bioactive compounds from kenaf seed oil-in-water nanoemulsions.
• •
  The particle size and structural changes before and after in vitro digestion were also evaluated.
• •
  High released of antioxidants (tocopherol and total phenolics acids) from the digested nanoemulsion.
• •
  Confocal micrograph has suggested better absorption and penetration rate.

Abstract

Kenaf (Hibiscus cannabinus L.) seed oil contains high nutrition value, suggesting its potential applications in food and nutraceutical fields. However, the poor water solubility and stability strictly limit its applications. A good physical stability of kenaf seed oil-in-water nanoemulsions stabilised by sodium caseinate, Tween 20 and β-cyclodextrin complexes were produced using high pressure homogeniser. A simple model of two-stage dynamic in vitro digestion was employed to investigate the releasing rate of bioactive compounds from kenaf seed oil-in-water nanoemulsions, compared to unencapsulated bulk oil. The particles size and structural changes during digestion were also evaluated. Digested nanoemulsions showed good lipid digestion (85.25%), good bioaccessibility of antioxidants (tocopherols and total phenolic contents) and lower degradation rate of phytosterols compared to digested bulk oil. This study provides good information about the characteristic and release behaviour of formulated kenaf seed oil-in-water nanoemulsions, which is important for the future application in food and nutraceutical industries.

Keywords

• Kenaf (Hibiscus cannabinus L.) seed oil
• Lipolysis
• Releasing behaviour
• Confocal microscopy
• Tocopherols
• Phytosterols

Abbreviations

• O/W, oil-in-water
• SC, sodium caseinate
• T20, Tween 20
• β-CD, β-cyclodextrin
• PDI, polydispersity index
• DPPH, 2,2-diphenyl-1-picrylhydrazyl
• TPC, total phenolic content
• FFA, free fatty acids
• SGF, simulated gastric fluid
• SIF, simulated intestinal fluid

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• ∗ 
  Corresponding author.

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