A novel detoxifying agent: Using rice husk carriers to immobilize zearalenone-degrading enzyme from Aspergillus niger FS10

Published Date
October 2016, Vol.68:271–279, doi:10.1016/j.foodcont.2016.03.042

• Mengling He ^a
• Yun Li ^a,b
• Fuwei Pi ^a
• Jian Ji ^a
• Xingxing He ^a
• Yinzhi Zhang ^a
• Xiulan Sun ^a,,

• aState Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, Jiangsu 214122, China
• bInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Beijing 100081, China

Received 25 December 2015. Revised 10 March 2016. Accepted 25 March 2016. Available online 28 March 2016.

Highlights

• A novel detoxifying agent for removing ZEN by immobilizing ZEN-degrading enzyme on rice husk is fabricated.
• •
  The ZEN-degrading enzyme manifests relatively good stability, thermostability compared with the native enzyme.
• •
  The removal efficiency of the produced detoxifying agent exerts admirably as respect to the commercial detoxifying agents.

Abstract

The greatest hidden peril for commercially detoxifying agents is that although a toxin binding to the adsorbent is discharged outside of the animal without causing damage to them, because the toxin is too stable to destroy, they accumulate in the soil or barn, and become secondary hazards to the environment and animals. In this study, a novel detoxifying agent which uses rice husk (RH) to immobilize zearalenone-degrading enzyme (ZDE) was produced to reduce zearalenone (ZEN). In addition, the ZDE exhibited high enhancement of both its stability and catalytic activity for removing ZEN. The immobilized ZDE is more resistant to thermal denaturation than the native enzyme. At 90 °C, it retained 70% ZEN removal rate compared to the native enzyme. Additionally, it was also more stable during storage at 4 °C and 25 °C: after one month the immobilized ZDE retained 90% and 70% of initial activity, respectively. Furthermore, a comparison of the removal rate of immobilized ZDE with other detoxifying agents in artificial porcine gastrointestinal digestive juice revealed that the ZEN removal efficacy of immobilized ZDE was higher than other detoxifying agents. Specifically, the removal efficacy of the immobilized ZDE was 75.45% for ZEN in gastric digestive juice and 90.43% in intestinal digestive juice.

Keywords

Detoxifying agent

Immobilization

Zearalenone

Rice husk

Artificial porcine gastrointestinal digestive juice

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 Table 1

Table 1.

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  Corresponding author.
  
  

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