Determination of ascorbic acid using CdTe quantum dots immobilized on eggshell membrane surface as a turn-on fluorescence probe

Published Date
December 2016, Vol.180:146–150, doi:10.1016/j.jlumin.2016.08.026

Author 

• Gao Xue 
• Zhao Yue
• Zhang Bing
• Tang Yiwei
• Liu Xiuying
• Li Jianrong ^,

• College of Chemistry, Chemical Engineering and Food Safety, Bohai University, Jinzhou 121013, People׳s Republic of China

Received 26 October 2015. Revised 10 August 2016. Accepted 12 August 2016. Available online 20 August 2016. 

Abstract

Eggshell membrane is a natural material, essentially made up of protein fibers having flexibility in the aqueous solution and possessing gas and water permeability. It was used as a biomembrane for immobilization of CdTe quantum dots (QDs) for the development of a simple and sensitive ascorbic acid (AA) biosensor. The layer-by-layer self-assembly technology was used to prepare the multilayer films via the electrostatic interaction between poly(dimethyldiallyl ammonium chloride) (PDDA) and CdTe QDs. Due to the efficient electron-transfer between CdTe QDs and Cu²⁺ions, the fluorescence of CdTe QDs was greatly quenched by Cu²⁺ ions. Upon addition of AA, Cu²⁺ was reduced to Cu⁺ and the interaction of Cu²⁺- CdTe QDs was destroyed, which inhibit the quenching and thus leading to fluorescence recovery of CdTe QDs. Experimental results showed that the recovery in fluorescence intensity was linearly proportional to the concentration of AA in the range of 10⁻¹² to 10⁻⁵ mol/L with a detection limit (LOD) of 4.38×10⁻¹³ mol/L under the optimized experimental conditions. The proposed turn-on fluorescence probe was successfully applied for the determination of AA in real samples with satisfactory reproducibility and accuracy. Due to good stability, environmental friendly, cheapness, nontoxic, well behaved optical properties, and biocompatibility, the fabricated bioplatform has the promising future for practical applications.

Keywords

CdTe QDs

Eggshell membrane

Copper ions

Ascorbic acid

Fluorescence turn-on

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• ⁎ 
  Corresponding author.

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