Study on the bio-methane yield and microbial community structure in enzyme enhanced anaerobic co-digestion of cow manure and corn straw

Published Date
November 2016, Vol.219:150–157, doi:10.1016/j.biortech.2016.07.116

• Xuemei Wang ^a
• Zifu Li ^a,,
• Xiaoqin Zhou ^a
• Qiqi Wang ^a
• Yanga Wu ^b
• Mayiani Saino ^a
• Xue Bai^a

• aSchool of Energy and Environmental Engineering, Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China
• bUniversity College London, UK

Received 27 June 2016. Revised 25 July 2016. Accepted 27 July 2016. Available online 28 July 2016.

Highlights

• Enzymatic treatment could improve anaerobic co-digestion of cow manure and corn straw.
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  The direct addition of amylase increased methane yield by 110.79%.
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  The cellulase pretreatment increased methane yield by 103.20%.
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  The enzymes slightly affected the structure of hydrolysis bacteria community.
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  Amylase, cellulase and protease had no harmful effect on microbial community.

Abstract 

The use of enzymes to improve anaerobic co-digestion (AcoD) of cow manure and corn straw was explored in this study, including cellulase pretreatment and direct additions of amylase and protease. The effects of enzymes on microbial community structure were investigated though PCR-DGGE method. Results showed that AcoD with amylase achieved the highest methane yield of 377.63 ml·CH₄/g·VS, which was an increase of 110.79%. The methane increment consumed the amylase of 4.18 × 10⁻⁵ g/ml·CH₄. Enzymes mainly affected the bacteria in the hydrolysis stage rather than the bacteria in the hydrogenesis and acetogenesis stage and the archaea in the methanogenesis stage. However, the experimental results demonstrated that enzymes had no negative influence on microbial communities; the predominant microbial communities were similar. Therefore, AcoD with amylase was an effective way to improve the bio-methane yield of cow manure and corn straw.

Keywords

Anaerobic co-digestion

Enzymatic treatment

Methane yield

PCR-DGGE

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  Corresponding author.
  
  

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