Synthesis and characterization of anti-bacterial and anti-fungal citrate-based mussel-inspired bioadhesives

Published Date
Biomaterials
April 2016, Vol.85:204–217, doi:10.1016/j.biomaterials.2016.01.069

Author 

• Jinshan Guo ^a,1
• Wei Wang ^a,b,1
• Jianqing Hu ^a,c,1
• Denghui Xie ^d
• Ethan Gerhard ^a
• Merisa Nisic ^a
• Dingying Shan ^a
• Guoying Qian ^b
• Siyang Zheng ^a
• Jian Yang ^a,,

• aDepartment of Biomedical Engineering, Materials Research Institute, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA
• bZhejiang Provincial Top Key Discipline of Bioengineering, College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
• cSchool of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China
• dDepartment of Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Academy of Orthopedics of Guangdong Province, Guangzhou 510630, China

Received 16 December 2015. Revised 27 January 2016. Accepted 31 January 2016. Available online 2 February 2016. 

Abstract

Bacterial and fungal infections in the use of surgical devices and medical implants remain a major concern. Traditional bioadhesives fail to incorporate anti-microbial properties, necessitating additional anti-microbial drug injection. Herein, by the introduction of the clinically used and inexpensive anti-fungal agent, 10-undecylenic acid (UA), into our recently developed injectable citrate-based mussel-inspired bioadhesives (iCMBAs), a new family of anti-bacterial and anti-fungal iCMBAs (AbAf iCs) was developed. AbAf iCs not only showed strong wet tissue adhesion strength, but also exhibited excellent in vitro cyto-compatibility, fast degradation, and strong initial and considerable long-term anti-bacterial and anti-fungal ability. For the first time, the biocompatibility and anti-microbial ability of sodium metaperiodate (PI), an oxidant used as a cross-linking initiator in the AbAf iCs system, was also thoroughly investigated. Our results suggest that the PI-based bioadhesives showed better anti-microbial properties compared to the unstable silver-based bioadhesive materials. In conclusion, AbAf iCs family can serve as excellent anti-bacterial and anti-fungal bioadhesive candidates for tissue/wound closure, wound dressing, and bone regeneration, especially when bacterial or fungal infections are a major concern.

Keywords

Wound closure

Antibacterial

Antifungal

Citric acid

Sodium metaperiodate

Silver nanoparticles

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• DOCX (12.8M)

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  Corresponding author. W340 Millennium Science Complex, University Park, PA 16802, USA.

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