Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring

Published Date
Biosensors and Bioelectronics
15 November 2005, Vol.21(5):742–748, doi:10.1016/j.bios.2005.01.008

• Author 

• Inna S. Alpeeva ^a
• Mihaela Niculescu-Nistor ^b
• Jaime Castillo Leon ^b
• Elisabeth Csöregi ^b
• Ivan Yu. Sakharov ^a,c,,

• aDepartment of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119992, Russia
• bDepartment of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
• cDivision of Chemistry, G.V. Plekhanov Russian Economic Academy, Stremyanny per. 28, Moscow 113054, Russia

Received 28 October 2004. Revised 11 January 2005. Accepted 12 January 2005. Available online 3 February 2005. 

Abstract

Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H₂O₂-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency, I_max,  , detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H₂O₂. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.

Keywords

Peroxidase

Biosensors

Palm tree

Horseradish

Sweet potato

Hydrogen peroxide

---

Fig. 1.

Fig. 2.

Fig. 3.

 Table 1

Table 1.

 Table 2

Table 2.

Fig. 4.

Fig. 5.

• * 
  Corresponding author. Tel.: +7 095 9393407; fax: +7 095 9392742.

Copyright © 2005 Elsevier B.V. All rights reserved.

For further details log on website :
http://www.sciencedirect.com/science/article/pii/S0956566305000230