Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk

Published Date
Renewable Energy
July 2017, Vol.107:36–41, doi:10.1016/j.renene.2017.01.037

• Author 

• Syarilaida Zulkefli ^a
• Emilia Abdulmalek ^a,b,,
• Mohd. Basyaruddin Abdul Rahman ^a,b

• aDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia
• bEnzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia

Received 15 April 2016. Revised 6 January 2017. Accepted 19 January 2017. Available online 21 January 2017. 

Highlights

• •
  Utilization of deep eutectic solvent (DES) in pretreatment of biomass provides a safe process.
• •
  Maximum dissolution (58%) of oil palm trunk (OPT) was achieved in DES of ethylammonium chloride: ethylene glycol (EAC:EG).
• •
  EAC:EG pretreated OPT has reduced lignin and hemicellulose content with an increase in cellulose content and shown the best glucose production.

Abstract

Deep eutectic solvents (DES) were utilised as pretreatment media on oil palm trunk (OPT) fibre in order to change the morphology of the highly crystalline cellulose prior to enzymatic hydrolysis. Among all the DESs tested, ethylammonium chloride: ethylene glycol (EAC:EG) was found to be the most efficient solvent for the pretreatment of OPT fibre. The pretreatment of OPT by EAC:EG had removed 42% lignin and 83% hemicellulose with the ability to dissolve the OPT biomass (58%) after heating at 100 °C for 48 h. FTIR analysis was used in determining the chemical characteristic changes where OPT treated in EAC:EG had more disruption of hydroxyl bond and indication of delignification compared to OPT treated with other DESs. Enzymatic hydrolysis was carried out on OPT treated in EAC:EG and the highest glucose production (74%) was achieved at 50 °C after 24 h with 15 mg/mL substrate concentration, 50 FPU/g of Celluclast 1.5 L and 100 CBU/ml of Novozyme 188.

Keywords

Deep eutectic solvent

Lignocellulosic biomass

Oil palm trunk

Pretreatment

Enzymatic hydrolysis

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• ∗ 
  Corresponding author. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

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