Research members： Makoto Yoshida PhD.
Summary
Wood preservatives are used to protect wood materials from deterioration by wood rotting fungi. Their mechanisms of action are normally based on the toxic compounds for various microorganisms, and thus, the development of next-generation preservatives which shows high specificity to wood decay would be needed from the viewpoint of environmental impact and public health.
Recently, we found a novel cellulose-binding pyranose dehydrogenase (PDH), and showed that PDH required pyrroloquinoline quinone (PQQ) as a cofactor. Since PQQ is well known to be produced only by limeted bacterial species, the transfer of PQQ from bacteria to fungi would be important to triger the catalytic reaction of this type of enzymes. In the present study, we have attempted to develop wood preservatives which shows high specificity to wood decay phenomena based on block of PQQ-transfer pathway from bacteria to fungi. The research is financially supported by JSPS KAKENHI Grant-in-Aid for Scientific Research (B) [Grant no.15H04526].
For further details log on website:
http://www.rd.tuat.ac.jp/en/activities/factors/search/20150730_3.html
Research fields： Forest and forest products science
Departments： Institute of Agriculture
Keywords： biomass conversion, wood protection, wood rotting fungi, biofuel, cellulose, hemicellulose, lignin
Summary
Wood preservatives are used to protect wood materials from deterioration by wood rotting fungi. Their mechanisms of action are normally based on the toxic compounds for various microorganisms, and thus, the development of next-generation preservatives which shows high specificity to wood decay would be needed from the viewpoint of environmental impact and public health.
Recently, we found a novel cellulose-binding pyranose dehydrogenase (PDH), and showed that PDH required pyrroloquinoline quinone (PQQ) as a cofactor. Since PQQ is well known to be produced only by limeted bacterial species, the transfer of PQQ from bacteria to fungi would be important to triger the catalytic reaction of this type of enzymes. In the present study, we have attempted to develop wood preservatives which shows high specificity to wood decay phenomena based on block of PQQ-transfer pathway from bacteria to fungi. The research is financially supported by JSPS KAKENHI Grant-in-Aid for Scientific Research (B) [Grant no.15H04526].
Reference articles and patents
1) Kiwamu Umezawa, Kouta Takeda, Takuya Ishida, Naoki Sunagawa, Akiko Makabe, Kazuo Isobe, Keisuke Koba, Hiroyuki Ohno, Masahiro Samejima, Nobuhumi Nakamura, Kiyohiko Igarashi, Makoto Yoshida*. A novel pyrroloquinoline quinone-dependent 2-keto-D-glucose dehydrogenase from Pseudomonas aureofaciens. Journal of Bacteriology 197, 1322-1329 (2015)
2) Kouta Takeda, Hirotoshi Matsumura, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida*, Kiyohiko Igarashi, and Nobuhumi Nakamura. Characterization of a Novel PQQ-Dependent Quinohemoprotein Pyranose Dehydrogenase from Coprinopsis cinerea Classified into Auxiliary Activities Family 12 in Carbohydrate-Active Enzymes. PLOS ONE 10, e0115722 (2015)
3) Hirotoshi Matsumura, Kiwamu Umezawa, Kouta Takeda, Naohisa Sugimoto, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida*, Kiyohiko Igarashi, Nobuhumi Nakamura. Discovery of a eukaryotic pyrroloquinoline quinone-dependent oxidoreductase belonging to a new auxiliary activity family in the database of carbohydrate-active enzymes. PLOS ONE 9, e104851 (2014)
Contact
University Research Administration Center(URAC),
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
(Please replace [at] with @.)
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
(Please replace [at] with @.)
For further details log on website:
http://www.rd.tuat.ac.jp/en/activities/factors/search/20150730_3.html
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