Zn2+, a key factor of colony morphogenesis of Tricholoma matsutake

Published Date

First online: 19 July 2016

Title 

Zn2+, a key factor of colony morphogenesis of Tricholoma matsutake

• Author 

• Tokuya Tasaki 
• , Ryuichiro Kondo

Abstract

Tricholoma matsutake is a basidiomycete producing edible fruiting bodies known as matsutake in Japanese. Matsutake is one of the most valuable edible mushrooms in Japan. Colony morphology on agar plates of T. matsutake is known to vary depending on compositions of media used, while the mechanism is still unknown. In our present work, we have found Zn2+ affects colony morphology of T. matsutake. When Zn2+ was added at concentration more than 1 µM, colony got wrinkled and aerial hyphae formation was activated. No such morphological changes have been confirmed without Zn2+addition or at concentration less than 1 µM, even when other nutritional ingredients are provided sufficiently. None of the Zn2+ concentrations examined present has affected colony diameter, dry weight, and water content. These findings imply Zn2+ is a key factor involved in colony morphogenesis of T. matsutake such as colony wrinkling and aerial hyphae formation. Metabolomic information obtained with gas chromatography–mass spectrometry has indicated that Zn2+ deficiency has influences on carbohydrate and l-serine metabolisms.

References

1. 1.
   Arai Y, Takao M, Sakamoto R, Yoshikawa K, Terashita T (2003) Promotive effect of the hot water-soluble fraction from corn fiber on vegetative mycelial growth in edible mushrooms. J Wood Sci 49:437–443CrossRef
2. 2.
   Guerin-Laguette A, Vaario LM, Matsushita N, Shindo K, Suzuki K, Lapeyrie F (2003) Growth stimulation of a Shiro-like, mycorrhiza forming, mycelium of Tricholoma matsutake on solid substrates by non-ionic surfactants or vegetable oil. Mycol Prog 2(1):37–44CrossRef
3. 3.
   Kawagishi H, Hamajima K, Takanami R, Nakamura T, Sato Y, Akiyama Y, Sano M, Tanaka O (2004) Growth promotion of Mycelia of Matsutake Mushroom Tricholoma matsutake by d-isoleucine. Biosci Biotechnol Biochem 68(11):2405–2407CrossRefPubMed
4. 4.
   Vaario LM, Guerin-Laguette A, Matsushita N, Suzuki K, Lapeyrie F (2002) Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants. Mycorrhiza 12:1–5CrossRefPubMed
5. 5.
   Kusuda M, Ueda M, Miyatake K, Terashita T (2008) Characterization of the carbohydrase productions of an ectomycorrhizal fungus, Tricholoma matsutake. Mycoscience 49:291–297CrossRef
6. 6.
   Ogawa M (1978) Matsutake no seibutsugaku (in Japanese). Tsukiji Shokan, Japan
7. 7.
   Geoffrey MG, Lynn R, John WG, Karl R (2001) Nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. FEMS Microbiol Lett 204:311–316CrossRef
8. 8.
   Ohta A (1990) A new medium for mycelial growth of mycorrhizal fungi. Trans Mycol Soc Jpn 31:323–334
9. 9.
   Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497CrossRef
10. 10.
    Marx DH (1969) The influence of ectotrophic mycorrhizal fungi on the resistance of pathogenic infections. I. Antagonism of mycorrhizal fungi to pathogenic fungi and soil bacteria. Phytopathology 59:153–163
11. 11.
    Talbot NJ (1997) Fungal biology: growing into the air. Curr Biol 7(2):78–81CrossRef
12. 12.
    Wessels JGH, De Vries OMH, Ásgeirsdóttir SA, Springer J (1991) The thn mutation of Schizophyllum commune, which suppresses formation of aerial hyphae, affects expression of the Sc3 hydrophobin gene. J Gen Microbiol 137:2439–2445CrossRefPubMed
13. 13.
    Vatsala TM, Shanmugasundaram KR, Shanmugasundaram ERB (1976) Role of nicotinic acid in pyridoxine biosynthesis in Aspergillus nidulans. Biochem Biophys Res Commun 72(4):1570–1575CrossRefPubMed
14. 14.
    Lanfranco L, Balsamo R, Martino E, Perotto S, Bonfante P (2002) Zinc ions alter morphology and chitin deposition in an ericoid fungus. Eur J Histochem 46:341–350CrossRefPubMed

For further details log on website :
http://link.springer.com/article/10.1007/s10086-016-1571-6