Development of 18 microsatellite markers in Pieris japonica, a poisonous tree insulated from the browsing pressure of herbivores, using a next-generation sequencer

Published Date
February 2015, Volume 20, Issue 1, pp 244–247

Title 

Development of 18 microsatellite markers in Pieris japonica, a poisonous tree insulated from the browsing pressure of herbivores, using a next-generation sequencer

• Author 

• Hiroyuki Kurokochi, 
• Engkong Tan, 
• Shuichi Asakawa, 
• Chunlan Lian

Short Communication

First Online: 

26 July 2014

DOI: 10.1007/s10310-014-0456-z

Cite this article as: 

Kurokochi, H., Tan, E., Asakawa, S. et al. J For Res (2015) 20: 244. doi:10.1007/s10310-014-0456-z

Abstract

Pieris japonica is a poisonous tree species that is rarely eaten by herbivorous animals, which could allow it to expand its distribution and change ecosystems. Using a next-generation sequencer, 18 microsatellite markers were isolated from P. japonica and characterized. The number of alleles at each locus ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.13 to 1.00 and 0.32 to 0.88, respectively. These markers will be useful for genetic studies of P. japonica, which will be essential for conservation of its surrounding environment.

References

1. Fujiki D, Kishimoto Y, Sakata H (2010) Assessing decline in physical structure of deciduous hard wood forest stands under sika deer grazing using shrub-layer vegetation cover. J For Res 15:140–144CrossRef
2. Hester AJ, Edenius L, Buttenschon RM, Kuiters AT (2000) Interactions between forests and herbivores: the role of controlled grazing experiments. Forestry 73:382–391CrossRef
3. Iwai N, Shoda-Kagaya E, Hamaguchi K (2012) Isolation and characterization of eight microsatellite loci in the Otton frog, Babina subaspera. J For Res 17:446–448CrossRef
4. Kurata S (1964) Illustrated important forest trees of Japan, vol 3. Chikyusha, Japan
5. Kurokochi H, Hogetsu T (2014) Fine-scale initiation of non-native Robinia pseudoacaciariparian forests along the Chikumagawa River in central Japan. J Ecol Environ 37:21–29CrossRef
6. Kurokochi H, Saito Y, Chuman M, Ide Y (2013) Low chloroplast diversity despite of phylogenetically divergent haplotypes in Japanese populations of Ailanthus altissima(Simaroubaceae). Botany 91:148–154CrossRef
7. Kurokochi H, Tan E, Asakawa S, Sukartiningsih, Saito Y, Ide Y (2014) Development of 16 microsatellite markers in Eusideroxylon zwageri by next-generation sequencing. Conserv Genet Resour. doi:10.1007/s12686-014-0148-1
8. Maesako Y, Nanami S, Kanzaki M (2007) Spatial distribution of two invasive alien species, Podocarpus nagi and Sapium sebiferum, spreading in a warm-temperate evergreen forest of the Kasugayama Forest Reserve, Japan. Veg Sci 24:103–112
9. Nomiya H, Suzuki W, Kanazashi T, Shibata M, Tanaka H, Nakashizuka T (2003) The response of forest floor vegetation and tree regeneration on deer exclusion and disturbance in a riparian deciduous forest, central Japan. Plant Ecol 164:263–276CrossRef
10. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
11. Sato M, Kurokochi H, Tan E, Asakawa S, Honda K, Bolisay K, Nakamura Y, Lian C, Fortes M, Nakaoka M (2014) Fifteen novel microsatellite markers for two Amphiprion species (Amphiprion frenatus and Amphiprion perideraion) and cross-species amplification. Conserv Genet Resour. doi:10.1007/s12686-014-0182-z
12. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRef
13. Suzuki M, Miyashita T, Kabaya H, Ochiai K, Asada M, Tange T (2008) Deer density affects ground-layer vegetation differently in conifer plantations and hardwood forests on the Boso Peninsula, Japan. Ecol Res 23:151–158CrossRef
14. Uehara K (1959) Illustrations of trees. Ariake Shobou, Tokyo
15. Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JA (2007) Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res 35:W71–W74PubMedCentralPubMedCrossRef
16. Vazwuez DP (2002) Multiple effects of introduced mammalian herbivores in a temperate forest. Biol Invasions 4:175–191CrossRef
17. Yokoyama S, Shibata E (1998) The effect of sika-deer browsing on the biomass and morphology of a dwarf bamboo, Sasa nipponica in Mt. Ohdaigaharam, central Japan. For Ecol Manag 103:49–56CrossRef

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