Phosphatidylinositol 3-phosphate, an essential lipid in Phytophthora sojae, enriches in the haustoria during infection

Published Date
August 2016, Volume 45, Issue 4, pp 435–441

Title 
Phosphatidylinositol 3-phosphate, an essential lipid in Phytophthora sojae, enriches in the haustoria during infection

Author 

Linlin Chen, 

Wen Wang, 

Ying Hou, 

Yuren Wu, 

Honglian Li, 

Daolong Dou

Article

First Online: 

08 July 2016

DOI: 10.1007/s13313-016-0430-7

Cite this article as: 

Chen, L., Wang, W., Hou, Y. et al. Australasian Plant Pathol. (2016) 45: 435. doi:10.1007/s13313-016-0430-7

Abstract

Oomycete pathogens, such as Phytophthora sojae, encode several hundreds of RXLR effectors to manipulate host immunity responses. For these effectors transporting into the host cells, P. sojae may produce PtdIns(3)P to bind the effectors. Transgenic P. sojae that expressed PtdIns(3)P-specific fluorescent probes were used for PtdIns(3)P distribution in parasite. Fluorescence was associated mainly with the endoplasmic reticulum (ER), where effectors might to bind PtdIns(3)P for transport. Furthermore, PtdIns(3)P biosensor was detected that enriched in haustoria during infection. We also identified three PsPI3Ksfunctionally complement yeast VPS34, which generates only PtdIns(3)P. These results further demonstrated that P. sojae might generated PtdIns(3)P to benefit their infectious process.

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