Technique IRAP (inter retrotransposon amplified polymorphism) to study the genetic variability in Sclerotinia sclerotiorum populations

Published Date
August 2016, Volume 45, Issue 4, pp 357–360

Title 
Technique IRAP (inter retrotransposon amplified polymorphism) to study the genetic variability in Sclerotinia sclerotiorum populations

Author 

Míriam Goldfarb, 

Mateus Ferreira Santana, 

Tania Maria Fernandes Salomão, 

Pedro Henrique Scarpelli Pereira, 

Marisa Vieira de Queiroz, 

Everaldo Gonçalves de Barros

Original Paper

First Online: 

10 June 2016

DOI: 10.1007/s13313-016-0421-8

Cite this article as: 

Goldfarb, M., Santana, M.F., Salomão, T.M.F. et al. Australasian Plant Pathol. (2016) 45: 357. doi:10.1007/s13313-016-0421-8

Abstract

Sclerotinia sclerotiorum, the causal agent of the white mold in dry beans, is responsible among other damages, for pronounced losses in bean cultivation. Therefore, there is an urgent need for greater research into the genetic variability of S. sclerotiorum. This study was done to evaluate the technical efficiency of IRAP (Inter Retrotransposon Amplified Polymorphism) to explore the genetic variability in this fungus. Primers were designing for the conserved regions of the long terminal repeats (LTRs) of the retrotransposons Copia-LTR_SS and Gypsy-LTR_SS. The primers were tested in the pathogen populations of S. sclerotiorum from Minas Gerais, Brazil. The estimated values of the high genetic variability indicate that the IRAP technique is a viable alternative and useful in population studies on the genetic variability of S. sclerotiorum.

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