Phytochemical analysis, cytotoxic activity and constituents-activity relationships of the leaves of Cinnamomum iners (Reinw. ex Blume-Lauraceae).

Published Date
Nat Prod Res. 2012;26(22):2155-8. doi: 10.1080/14786419.2011.633083. Epub 2011 Dec 19.

Title 

Phytochemical analysis, cytotoxic activity and constituents-activity relationships of the leaves of Cinnamomum iners (Reinw. ex Blume-Lauraceae).

Ghalib RM1, Hashim R, Sulaiman O, Mehdi SH, Anis Z, Rahman SZ, Ahamed BM, Abdul Majid AM.

Author information

• 1School of Industrial Technology, Universiti Sains Malaysia, Minden - 11800, Pulau Pinang, Malaysia. raza2005communications@gmail.com

Abstract

The leaves of Cinnamomum iners (Reinw. ex Blume-Lauraceae) have been refluxed successively with chloroform and alcohol to get chloroform extract and alcoholic extract. Both the extracts have been assayed for cytotoxicity against human colorectal tumour cells. The chloroform extract exhibited significant cytotoxicity with IC(50) 31 µg mL(-1) (p < 0.01). However, ethanol extract was found to be much less cytotoxic with IC(50) > 200 µg mL(-1). The chloroform extract has been further proceeded for chemical analysis by GC-TOFMS and 178 components were identified including acids, amines, amides, aldehydes, alcohols, esters, benzene derivatives, bicyclic compounds, terpenes, hydrocarbons, naphthalene derivatives, furan derivatives, azulenes, etc. Nine components representing 51.73% of the total chloroform extract were detected as major components. Caryophyllene (14.41%) and Eicosanoic acid ethyl ester (12.17%) are the most prominent components of the chloroform extract. β-Caryophyllene (14.41%) as most abundant compound supports potent cytotoxicity as shown by chloroform extract.

For further details log on website :
http://www.ncbi.nlm.nih.gov/pubmed/22181707