Preliminary study on enzymatic hydrolysis of treated oil palm (Elaeis) empty fruit bunches fibre (EFB) by using combination of cellulase and β 1-4 glucosidase

Published Date
March 2011, Vol.35(3):1055–1059, doi:10.1016/j.biombioe.2010.11.020

Author

• Fazlena Hamzah
 
• Ani Idris ^,
 
• Tan Khai Shuan

• Department of Bioprocess Engineering, Faculty of Chemical Engineering and Natural Resources Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia

Received 26 August 2008. Revised 2 November 2010. Accepted 8 November 2010. Available online 3 December 2010.

Abstract

Preliminary study on enzymatic hydrolysis process using combination of cellulase and β 1-4 glucosidase on treated oil palm empty fruit bunch fibre (EFB) was performed. Crucial trends for parameters such as pH, temperature and substrate loading influencing the enzymatic hydrolysis of the treated EFB fibre were also studied. Results revealed that a combination of both cellulase and β 1-4 glucosidase with the ratio of 5:1 hydrolyzed more cellulose from treated EFB fibre and gave highest soluble glucose concentration up to 4 g L⁻¹. The results indicated that as pH and temperature were increased the glucose produced also increased until pH 4.8 and 50 °C; beyond these values the reverse occurred. Glucose produced in the reaction increased with the increment in the substrate loading and maximum glucose concentration (2.7 g L⁻¹) was achieved when 8% (wv⁻¹) treated EFB was used as a substrate.

Keywords

EFB

Elaeis guineensis

Hydrolysis

Morphology

Cellulase

β 1-4 glucosidase

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