Published Date
2016, Vol.18:155–161, doi:10.1016/j.proche.2016.01.024
Molecular and Cellular Life Sciences: Infectious Diseases, Biochemistry and Structural Biology 2015 Conference
Open Access, Creative Commons license
Author
Corncob Oil Palm Empty Fruit Bunch (OPEFB) is an agricultural residue that has the potential to become a good source for renewable feedstock for production of sugar. This work evaluated the effectiveness of aqueous ammonia as pretreatment at low (soaking, SAA) and elevated temperature (Pressurized Chamber) to deconstruct the lignocellulosic feedstock, prior to enzymatic hydrolysis. The ammonia pretreatments were compared against the standard NaOH method. The best tested Pressurized Chamber method conditions were at 100 °C with 3 hour retention time, 12.5% Ammonium hydroxide and 1:30 solid loading. The digestibility of the feedstock is determined with enzymatic hydrolysis using Cellic Ctech2 and Cellic Htech2. The sugars produced by Pressurized Chamber method within 24 hour of enzyme hydrolysis are similar to that produced by NaOH method which is 439.90 mg/ml and 351.61 mg/ml, respectively. Compared with optimum SAA method (24 hour, 6.25% of ammonium hydroxide at room temperature), Pressurized Chamber method was capable of producing enhanced delignification and higher production of sugar upon hydrolysis. These findings were supported by the disappearance peak at 1732, 1512 and 1243 on Fourier Transform Infrared (FTIR spectrum) of treated OPEFB by Pressurized Chamber method. XRD determination showed reduced crystallinity of OPEFB (37.23%) after treatment by Pressurized Chamber, suggesting higher accessibility toward enzyme hydrolysis. The data obtained suggest that the Pressurized Chamber pre-treatment method are suitable for OPEFB deconstruction to produce high yield of sugar.
Keywords
Empty Fruit Bunch (EFB)
Pressurized chamber
Bio-mass conversion
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http://www.sciencedirect.com/science/article/pii/S1876619616000255
2016, Vol.18:155–161, doi:10.1016/j.proche.2016.01.024
Molecular and Cellular Life Sciences: Infectious Diseases, Biochemistry and Structural Biology 2015 Conference
Open Access, Creative Commons license
Author
Available online 11 February 2016.
AbstractCorncob Oil Palm Empty Fruit Bunch (OPEFB) is an agricultural residue that has the potential to become a good source for renewable feedstock for production of sugar. This work evaluated the effectiveness of aqueous ammonia as pretreatment at low (soaking, SAA) and elevated temperature (Pressurized Chamber) to deconstruct the lignocellulosic feedstock, prior to enzymatic hydrolysis. The ammonia pretreatments were compared against the standard NaOH method. The best tested Pressurized Chamber method conditions were at 100 °C with 3 hour retention time, 12.5% Ammonium hydroxide and 1:30 solid loading. The digestibility of the feedstock is determined with enzymatic hydrolysis using Cellic Ctech2 and Cellic Htech2. The sugars produced by Pressurized Chamber method within 24 hour of enzyme hydrolysis are similar to that produced by NaOH method which is 439.90 mg/ml and 351.61 mg/ml, respectively. Compared with optimum SAA method (24 hour, 6.25% of ammonium hydroxide at room temperature), Pressurized Chamber method was capable of producing enhanced delignification and higher production of sugar upon hydrolysis. These findings were supported by the disappearance peak at 1732, 1512 and 1243 on Fourier Transform Infrared (FTIR spectrum) of treated OPEFB by Pressurized Chamber method. XRD determination showed reduced crystallinity of OPEFB (37.23%) after treatment by Pressurized Chamber, suggesting higher accessibility toward enzyme hydrolysis. The data obtained suggest that the Pressurized Chamber pre-treatment method are suitable for OPEFB deconstruction to produce high yield of sugar.
Keywords
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- ☆Peer-review under responsibility of the organizing committee of the Molecular and Cellular Life Sciences: Infectious Diseases, Biochemistry and Structural Biology 2015 (MCLS 2015).
- ⁎ Corresponding author.
http://www.sciencedirect.com/science/article/pii/S1876619616000255
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