SpringerLink

Article

Folia Microbiologica

March 2016, Volume 61, Issue 2, pp 119-128

First online: 22 July 2015

Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymansgrown on spruce wood

• Jiří Gabriel 
• , Zdeněk Žižka
• , Karel Švec
• , Andrea Nasswettrová
• , Pavel Šmíra
• , Olga Kofroňová
• , Oldřich Benada

• 
  

• Look 

Abstract

This work describes autofluorescence of the mycelium of the dry rot fungus Serpula lacrymans grown on spruce wood blocks impregnated with various metals. Live mycelium, as opposed to dead mycelium, exhibited yellow autofluorescence upon blue excitation, blue fluorescence with ultraviolet (UV) excitation, orange-red and light-blue fluorescence with violet excitation, and red fluorescence with green excitation. Distinctive autofluorescence was observed in the fungal cell wall and in granula localized in the cytoplasm. In dead mycelium, the intensity of autofluorescence decreased and the signal was diffused throughout the cytoplasm. Metal treatment affected both the color and intensity of autofluorescence and also the morphology of the mycelium. The strongest yellow signal was observed with blue excitation in Cd-treated samples, in conjunction with increased branching and the formation of mycelial loops and protrusions. For the first time, we describe pink autofluorescence that was observed in Mn-, Zn-, and Cu-treated samples with UV, violet or. blue excitation. The lowest signals were obtained in Cu- and Fe-treated samples. Chitin, an important part of the fungal cell wall exhibited intensive primary fluorescence with UV, violet, blue, and green excitation.

Title

Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymans grown on spruce wood

Journal

Folia Microbiologica
Volume 61, Issue 2 , pp 119-128

Cover Date

2016-03

DOI

10.1007/s12223-015-0415-x

Print ISSN

0015-5632

Online ISSN

1874-9356

Publisher

Springer Netherlands

Additional Links

• Register for Journal Updates
• Editorial Board
• About This Journal
• Manuscript Submission

Authors

• Jiří Gabriel  (1)
• Zdeněk Žižka (2)
• Karel Švec (1)
• Andrea Nasswettrová (3)
• Pavel Šmíra (3)
• Olga Kofroňová (2)
• Oldřich Benada (2)

Author Affiliations

• 1. Laboratory of Environmental Microbiology, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic
• 2. Laboratory of Characterization of Molecular Structure, Institute of Microbiology AS CR, Vídeňská 1083, 142 20, Prague 4-Krč, Czech Republic
• 3. Research and Development, Thermo Sanace s.r.o., Chamrádova 475/23, 718 00, Ostrava-Kunčičky, Czech Republic

For further details log on website :

http://link.springer.com/article/10.1007%2Fs12223-015-0415-x