Preparation and properties of RS III from waxy maize starch with pullulanase

Published Date

August 2013, Vol.33(1):19–25, doi:10.1016/j.foodhyd.2013.02.018

Title 

Preparation and properties of RS III from waxy maize starch with pullulanase

• Author 

• Miaomiao Shi
• Yun Chen 
• Shujuan Yu
• Qunyu Gao ^,

• Carbohydrate Laboratory, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China

Received 12 October 2012. Accepted 26 February 2013. Available online 6 March 2013.

Abstract

Waxy maize starch was treated by pullulanase debranching and retrogradation at room temperature to produce resistant starch (RS). Physicochemical properties, crystalline structure and in-vitro digestibility of starch samples with different RS content were investigated. Compared with native starch, apparent amylose content of RS products increased. Based on Gel Permeation Chromatography (GPC) the Molecular Weight Distribution (MWD) of resistant starches significantly changed. Scanning Electron Microscopy (SEM) showed that upon pullulanase debranching and retrogradation treatment the granular structure of native starch was destroyed and all RS samples exhibited irregular shaped fragments. Crystal structure of samples changed from A–type to a mixture of B and V–type. The crystallinity of resistant starch also improved as compared with native starch. Moreover, samples with higher resistant starch showed higher relative crystallinity. Differential Scanning Calorimetry (DSC) determination showed that To、Tp、Tc and ΔH all increased which was in agreement with RS content. The resistance of waxy maize starch with Pullulanase treatment to α-amylase digestibility also increased, while the in-vitro digestibility of products decreased.

Graphical abstract

Highlights

► Waxy maize starch was treated by cooking, pullulanase debranching and retrogradation to produce resistant starch (RS). ► The molecular weight (MW) distribution of resistant starches significantly decreased. ► The digestion rate of resistant starch decreased with increasing incubation time.

Keywords

• Waxy maize starch
• Pullulanase
• Resistant starch
• Molecular weight distribution

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• ∗ 
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