Development of an efficient Tissue Culture after Crossing (TCC) system for transgenic improvement of barley as a bioenergy crop

Author

Satish Nalawade, Swati Nalawade, Chunlin Liu, Christer Jansson and Chuanxin Sun

Applied Energy, 2012, vol. 91, issue 1, pages 405-411

Abstract: We have developed a protocol for rapid generation of barley plants with introduced genes by employing techniques of tissue culture, plant regeneration and crossing. Compared to conventional protocols, the time between pollination of T0 plants and identification of phenotypic expression in the T1 generation is reduced from around 17–9weeks. All of the selected candidates after the tissue culture screening exhibited phenotypic expression. Furthermore, by utilizing ordinary pollen crossing, we demonstrated a route for introduction of genes to a recalcitrant barleyelite cultivar. This Tissue Culture after Crossing (TCC) procedure significantly enhances the prospects for producing transgenic barley lines from desirable germplasm. We suggest that the TCC method will contribute to improve barley as a promising bioenergy crop.

Keywords: Barley; Bioenergy; Tissue culture; Transgenics (search for similar items in EconPapers)
Date: 2012
References: View references in EconPapers View complete reference list from CitEc
Citations Track citations by RSS feed

Downloads: (external link)
http://www.sciencedirect.com/science/article/pii/S0306261911006490
Full text for ScienceDirect subscribers only

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link:http://EconPapers.repec.org/RePEc:eee:appene:v:91:y:2012:i:1:p:405-411

Ordering information: This journal article can be ordered from
http://www.elsevier.com/wps/find/journaldescription.cws_home/405891/bibliographic
http://www.elsevier. ... 405891/bibliographic

Access Statistics for this article

Applied Energy is currently edited by J. Yan

More articles in Applied Energy from  Elsevier
Series data maintained by Shamier, Wendy (repec@elsevier.com).

For further details log on website :
http://econpapers.repec.org/scripts/search.pf?kw=Barley