Isolation and characterization of a TERMINAL FLOWER 1 homolog from Prunus serotina Ehrh

Author

Publication Toolbox

• Download PDF (4.1 MB)
• This publication is available only online.

• Wang, Ying
• Pijut, Paula M.

Year Published

2013

Source

Tree Physiology. 33: 855-865.

Abstract

Flowering control is one of the several strategies for gene containment of transgenic plants. TERMINAL FLOWER 1 (TFL1) is known to be involved in the transcriptional repression of genes for inflorescence development. Two TFL1 transcripts with different 3' UTR were cloned from black cherry (Prunus serotinaEhrh.) using reverse transcription-polymerase chain reaction (RT–PCR) and rapid amplification of cDNA ends (RACE). Corresponding to the two TFL1 transcripts, two PsTFL1 gene sequences, 1248 bp and 1579 bp, were obtained and both contained the same 519 bp coding region which encoded a putative protein of 172 amino acid residues. The phylogenetic analysis of the amino acid sequences showed high identity of PsTFL1to TFL1 orthologs of other Prunus species, including Yoshino cherry (Prunus x yedoensis Matsum.), peach (Prunus persica (L.) Batsch), apricot (Prunus armeniaca L.) and Japanese apricot (Prunus mume Sieb. et Zucc.). The real-time quantitative PCR detected a single copy of PsTFL1 gene sequences in the black cherry genome with two alleles. The gene expression of PsTFL1 was examined in several tissues including the stems, leaves, shoot tips, and vegetative and floral buds. The highest mRNA level was detected in shoot tips, and the lowest level in the leaves. Transgenic Arabidopsis thaliana (L.) Heynh. plants overexpressing PsTFL1showed significantly delayed flowering. These plants also showed largely increased vegetative growth, plant height, number of nodes, trichome density, and the conversion of flower to shoot was observed at each node and shoot apex.

Keywords

• black cherry
• ectopic expression
• flowering
• Prunus serotina
• PsTFL1

Citation

Wang, Ying; Pijut, Paula M. 2013. Isolation and characterization of a TERMINAL FLOWER 1 homolog from Prunus serotina Ehrh. Tree Physiology. 33: 855-865.

Last updated on: October 23, 2013

For further details log on website :
https://www.nrs.fs.fed.us/pubs/45027