Isolation and molecular analysis of R-gene in resistant Zingiber officinale (ginger) varieties against Fusarium oxysporum f.sp. zingiberi

Author

R.Swetha PriyaR.B.Subramanian

BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Sattelite Campus, P.O. Box No. 39, Sardar Patel University, Vallabh Vidyanagar, 388 120 Gujarat, India

Received 2 November 2006, Revised 30 April 2007, Accepted 30 June 2007, Available online 4 September 2007.

Abstract

Marker assisted selection (MAS) of resistant varieties is a reliable and faster method of selecting the right varieties for cultivation. The aim of the present study is to find the genes responsible for resistance in highly resistant varieties. In the present work we report the presence of a Resistance (R) gene of CC–NBS–LRR class of plant resistance genes. Both direct PCR amplification from genomic DNA as well as cDNAs, yielded a 0.6 kb DNA sequence indicating the absence of an intron. Sequence analysis of the PCR amplicon obtained from the genomic DNA showed very high homology to R-genes. An interesting observation from the present study is the presence of the R-gene in only resistant varieties. Neither the partially resistant or susceptible varieties showed the presence of this gene sequence. This in turn raises interesting questions on the evolution of these ginger varieties. The cloned R-genes provide a new resource of molecular markers for rapid identification of fusarium yellows resistant ginger varieties.

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