Microsatellite Markers for Cariniana legalis (Lecythidaceae) and Their Transferability to C. estrellensis

Applications in Plant Sciences 1(6):1200493. 2013 
https://doi.org/10.3732/apps.1200493

Author

Evandro Vagner Tambarussi

, 

Alexandre Magno Sebbenn

, 

Maria Andréia Moreno

, 

Elza Martins Ferraz

, 

Paulo Yoshio Kageyama

, and 

Roland Vencovsky

Received: September 17, 2012; Accepted: November 18, 2012

; 

Published: May 7, 2013

[+] Author & Article Info

• Premise of the study: Microsatellite primers were developed in the neotropical tree speciesCariniana legalis (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow.
• Methods and Results: We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 C. legalis specimens and 26 C. estrellensis specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in C. legalis and three to 12 in C. estrellensis. For C. legalis, the observed (Ho) and expected (He) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For C. estrellensis,Hoand Heranged from 0 to 0.96 and from 0.14 to 0.91, respectively.
• Conclusions: The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in C. legalis and the related species C. estrellensis.

Keywords: Brazilian Atlantic forest, Cariniana legalis, conservation genetics, microsatellite markers, population genetics, tropical tree species

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This research was supported by Fundação de Amparo à Pesquisa do Estado da São Paulo (FAPESP; 2010/10704-7) and by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; 470491/2010-8). The authors would like to thank FAPESP for financial support provided to E.V.T. (scholarship no. 2010/12354-3) and CNPq for financial support to A.M.S. and R.V.

Cited by

Evandro Vagner Tambarussi, Lou C. Menezes, Bruna Ibañes, Lia Maris Orth Ritter Antiqueira, Gabriel Dequigiovanni, Maria Andréia Moreno, Elza Martins Ferraz, Maria Imaculada Zucchi, Elizabeth Ann Veasey, Roland Vencovsky. (2017) Microsatellite markers for Cattleya walkeriana Gardner, an endangered tropical orchid species. Plant Genetic Resources 15:01, 93-96.
Online publication date: 14-Jan-2016.
Crossref

Evandro Vagner Tambarussi, David Boshier, Roland Vencovsky, Miguel Luiz Menezes Freitas, Alexandre Magno Sebbenn. (2017) Inbreeding depression from selfing and mating between relatives in the Neotropical tree Cariniana legalis Mart. Kuntze. Conservation Genetics 18:1, 225-234.
Online publication date: 19-Oct-2016.
Crossref

E.V. Tambarussi, D.H. Boshier, R. Vencovsky, M.L.M. Freitas, O.J. Di-Dio and A.M. Sebbenn. (2016) Several Small: How Inbreeding Affects Conservation of Cariniana legalis Mart. Kuntze (Lecythidaceae) the Brazilian Atlantic Forest's Largest Tree. International Forestry Review 18:4, 502-510.
Online publication date: 4-Jan-2017.

Abstract & References : Full Text : PDF (133 KB) 

Lia Maris Orth Ritter Antiqueira, Gabriel Dequigiovanni, Evandro Vagner Tambarussi, Jucelene Fernandes Rodrigues, Elizabeth Ann Veasey. (2016) Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum. Crop Breeding and Applied Biotechnology 16:4, 355-360.
Online publication date: 1-Dec-2016.
Crossref

Maria Lucia Carneiro Vieira, Luciane Santini, Augusto Lima Diniz, Carla de Freitas Munhoz. (2016) Microsatellite markers: what they mean and why they are so useful. Genetics and Molecular Biology 39:3, 312-328.
Online publication date: 1-Sep-2016.
Crossref

Evandro V. Tambarussi, David Boshier, Roland Vencovsky, Miguel L. M. Freitas, Alexandre M. Sebbenn. (2015) Paternity analysis reveals significant isolation and near neighbor pollen dispersal in small Cariniana legalis Mart. Kuntze populations in the Brazilian Atlantic Forest. Ecology and Evolution 5:23, 5588-5600.
Online publication date: 13-Nov-2015.
Crossref

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