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Friday 19 August 2016

Transcriptome responses of grafted Citrus sinensis plants to inoculation with the arbuscular mycorrhizal fungus Glomus versiforme

Published Date
, Volume 30, Issue 4, pp 1073–1082

Title 

Transcriptome responses of grafted Citrus sinensis plants to inoculation with the arbuscular mycorrhizal fungus Glomus versiforme

  • Author 
  • Xuan Gao, 
  • Shuang Zhao, 
  • Qing-Long Xu, 

Original Article
First Online: 
09 March 2016
DOI: 10.1007/s00468-015-1345-6

Cite this article as: 
Gao, X., Zhao, S., Xu, Q. et al. Trees (2016) 30: 1073. doi:10.1007/s00468-015-1345-6

Abstract

Key message

‘Newhall’ grafted onto xiangcheng rootstock withGlomus versiformeor without displayed different responses, and genes related to photosystem II and alpha-linolenic acid metabolism pathways were involved in the responses.

Abstract

Previous studies have shown that there are significant differences in the physiological responses of ‘Newhall’ (Citrus sinensis) scions grafted onto trifoliate orange (Poncirus trifoliata) to arbuscular mycorrhizal (AM) fungi inoculation under normal and stress conditions. However, little is known about the transcriptomic responses of C. sinensis to AM fungi inoculation. In this study, we investigated the effects of inoculating the AM fungus Glomus versiforme on the biomass, pigment content, magnesium (Mg) content and distribution, net photosynthesis rate, and global transcriptome profile of ‘Newhall’ scions grafted onto xiangcheng (Citrus junos) rootstock. The results showed that AM inoculation significantly increased plant growth, Mg concentration, and photosynthesis, but not pigment contents. More than 68,299,008 transcripts were examined in spring-flush leaves, and 29 genes were identified as being differentially expressed in response to mycorrhizal colonization. The differentially expressed genes encoded proteinase inhibitors, transporters, and products related to chlorophyll and disease resistance. Genes encoding proteins related to chlorophyll and transport were up-regulated by AM inoculation while genes encoding proteinase inhibitors were down-regulated. Gene Ontology and KEGG database analyses revealed that genes related to photosystem II and alpha-linolenic acid metabolism pathways were involved in the response to AM inoculation. Comparative expression profiling revealed that the enhancement of photosynthesis after AM inoculation was due to activation of the light-harvesting complex family of proteins in photosystem II. Our results provide new insights into plant–mycorrhizal fungi interactions and their effects on plant growth.

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