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Thursday 1 September 2016

In planta transformation of kenaf plants (Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens.

Published Date
J Biosci Bioeng. 2004;98(2):136-9.

Title 
In planta transformation of kenaf plants (Hibiscus cannabinus var. aokawa No. 3) by Agrobacterium tumefaciens.

Author 
Kojima M1, Shioiri H, Nogawa M, Nozue M, Matsumoto D, Wada A, Saiki Y, Kiguchi K.

Abstract

Kenaf was transformed by inoculation of Agrobacterium tumefaciens onto the meristems of young plants in pots. The transformation was demonstrated by three lines of evidence: a phenotypic inheritance from T(0) to T(1) plants, detection of the transgene in both T(0) and T(1) plants, and rescue of plasmids composed of T-DNA of the binary vector and flanking plant genomic DNA from T(1) plants.


For further details log on website :
http://www.ncbi.nlm.nih.gov/pubmed/16233680

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