Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria

Published Date
30 December 2015, Vol.78:148–153, doi:10.1016/j.indcrop.2015.10.015

Author

• Lila Harkat-Madouri ^a,b
• Boudria Asma ^a
• Khodir Madani ^a
• Zakia Bey-Ould Si Said ^a
• Peggy Rigou^c
• Daniel Grenier ^d
• Hanane Allalou ^a
• Hocine Remini ^a
• Abdennour Adjaoud ^a
• Lila Boulekbache-Makhlouf ^a,,

• aLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria
• bFaculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene, Alger, Algeria
• cINRA, UMR 1083 Sciences pour l’oenologie, Montpellier, France
• dGroupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Québec, Québec, Canada

Received 13 July 2015. Revised 7 October 2015. Accepted 7 October 2015. Available online 16 November 2015. 

Highlights

• 26 volatile compounds have been detected in essential oil of leaves E. globulus.
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  1,8-Cineole being the main component (55.29%).
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  Essential oil of leaves E. globulus exhibited a weak antioxidant capacity.
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  The tested oil was more active on gram negative than on gram positive bacteria.
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  Eucalyptus globulus essential oils may have potential for oral care products.

Abstract

Essential oils are known for their use in various fields such as cosmetic, pharmaceutical and food industries. The aim of this work is to investigate the chemical composition of essential oils from Eucalyptus globulus leaves (E. globulus) by gas-chromatography coupled with mass spectrometry (GC/MS) method, and to evaluate their antioxidant capacity (DPPH radical scavenging effect, reducing power, and inhibition of lipid peroxidation activity) as well as their antibacterial activity, against periodontopathogenic and cariogenic bacterial species, using microdilution method in 96-well microplates. In total, 26 compounds were identified with the predominance of oxygenated monoterpenes (78.58%); 1,8-Cineole (55.29%), Spathulenol (7.44%) and α-Terpineol (5.46%) being the main components. The analyzed oils exhibited a weak antioxidant capacity, but a marked antibacterial activity against Gram negative bacteria, mainly for F. nucleatum ATCC 25586 (MIC = 1.14 mg/mL) and P. gingivalis ATCC33277 (MIC = 0.28 mg/mL). Therefore, E. globulus essential oils may have a potential therapeutic application for the treatment of periodontal diseases.

Keywords

Eucalyptus globulus

Essential oil

GC/MS analysis

Antibacterial activity

Antioxidant capacity

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  Corresponding author. Fax: +213 34 21 47 62.

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