Evaluation of wood treated with some natural extracts and Paraloid B-72 against the fungus Trichoderma harzianum: Wood elemental composition, in-vitro and application evidence

Published
May 2015, Vol.100:62–69, doi:10.1016/j.ibiod.2015.02.009

Author 

• Maisa M.A. Mansour ^a
• Mohamed Z.M. Salem ^b,,

• aConservation Department, Faculty of Archaeology, Cairo University, Giza 12613, Egypt
• bForestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt

Received 23 October 2014. Revised 6 February 2015. Accepted 9 February 2015. Available online 3 March 2015. 

Highlights

• Growth inhibition of Trichoderma harzianum by different natural extracts.
• •
  Morus alba heartwood and Maclura pomifera bark methanol extracts had highly significant effects.
• •
  Inhibition zones were shown around Acacia saligna wood treated with methanol extract of Cupressus sempervirens wood (CS).
• •
  ESEM and EDX surface wood analyses indicated the effect of treated wood with T. harzianum.
• •
  Paraloid B-72 combination with CS might be used as a potent biocide against the mold fungus T. harzianum.

Abstract

The damage in wood is commonly associated with fungi which do not affect wood strength. This study investigates the in-vitro growth inhibition of Trichoderma harzianum, a well-known fungus, by different methanol extracts from selected tree species. We observed the hyphal growth by the environmental scan electron microscope (ESEM) and evaluated the changes in the elemental composition by the electron dispersive X-ray spectroscopy (EDX). The methanolic extracts of Morus albaheartwood and Maclura pomifera bark had significant effects (P < 0.001) on the linear growth of T. harzianum. The treated wood samples of Acacia saligna with the methanolic extract of Cupressus sempervirens wood showed an inhibition zone against the growth of T. harzianum around the treated wood at the concentrations of 5, 10, and 20%. However, ESEM and EDX analyses of treated wood demonstrated that the combination of Paraloid B-72 and the methanolic extract of C. sempervirenswood might be used as a potent biocide against the mold fungus T. harzianum.

Keywords

Wood

Natural extracts

Trichoderma harzianum

Paraloid B-72

EDX

ESEM

Elemental composition

---

 Table 1

Table 1.

Fig. 1.

Fig. 2.

 Table 2

Table 2.

Fig. 3.

Fig. 4.

Fig. 5.

• ∗ 
  Corresponding author.
  
  

For further details log on website :
http://www.sciencedirect.com/science/article/pii/S0969806X15300402