Genetic Transformation in Citrus

Published Date
Volume 66 of the series Forestry Sciences pp 227-243

Author 

G. A. Moore

, D. Luth

, F. Kaplan

, M. A. Gutiérrez-E

Abstract

More Citrus is produced on a world-wide basis than any other fruit crop. The genus includes a number of species grown for fruit and/or juice products, including sweet oranges (C. sinensis [L.] Osbeck), grapefruit (C. paradisiMacf.), mandarins (C. reticulata Blanco), lemons (C. limon[L.] Burm. f.), and limes (C. aurantifolia [Christm.] Swing.) (Hodgson, 1967). Other species, such as sour orange (C. aurantium L.), are primarily grown for use as rootstocks, to which scions are budded. Citrus species are diploid with a relatively low chromosome number (n=9) and a small genome (1c=0.62 pg) (Guerra, 1984). They are grown throughout tropical and subtropical regions of the world.


References

1. An G (1987) Binary Ti vectors for plant transformation and promotor analysis. Methods Enzymol 153: 292–305CrossRef
2. Bar-Joseph M, Marcus R, Lee RF (1989) The continuous challenge of citrus tristeza virus control. Ann Rev Phytopath 27: 291–316CrossRef
3. Barrett HC, Rhodes AM (1976) A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Syst Bot 1: 105–136CrossRef
4. Bowman KD, Gmitter FG (1990) Forbidden fruit (Citrus sp., Rutaceae) rediscovered in Saint Lucia. Econ Bot 44: 165–173
5. Cervera M, Juarez J, Navarro A, Pina JA, Duran-Vila N, Navarro L, Pena L (1998) Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage. Transgenic Res 7: 51–59CrossRef
6. Fillatti JJ, Sellmer J, McCown B, Hassig B, Cornai L (1987) Agrobacterium-mediated transformation and regeneration of Populus. Mol Gen Genet 206: 192–199
7. Frost HB (1943) Genetics and breeding. In: Webber HJ, Batchelor LD (eds) The Citrus Industry, Vol 1, Univ of Calif Press, Berkeley, pp 817–913
8. Frost FEB, Soost RK (1968) Seed reproduction: Development of gametes and embryos. In: Reuther W, Batchelor LD, Webber HJ (eds) The Citrus Industry, Vol 2, University of California Press, Berkeley, pp 292–334
9. Gmitter FG, Moore GA (1986) Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: embryo production, germination, and plant survival. Plant Cell, Tissue and Organ Culture 6: 139–147.
10. Gmitter FG, Grosser JA, Moore GA (1992) Citrus. In: F. Hammerschlag and R. Litz (eds.) Biotechnology of Perennial Fruit Crops. CAB International, Wallingford, Oxon, UK, pp. 335–369.
11. Ghorbel R, Navarro L, Duran-Vila N (1998) Morphogenesis and regeneration of whole plants of grapefruit (Citrus paradisi), sour orange (C. aurantium) and alemow (C. macrophylla). J Hort Sci & Biotech 73: 323–327
12. Gross J (1977) Carotenoid pigments in citrus. In: Nagy S, Shaw PE, Veldhuis MK (eds) Citrus Science & Technology, AVI Publishing, Westport, CT, pp 302–354
13. Guerra MS. (1984) Cytogenetics of Rutaceae. II. Nuclear DNA content. Caryologia 37: 219–226
14. Gutiérrez-E. MA (1995) Production of transgenic citrus plants expressing the citrus tristeza virus coat protein gene. Ph.D Dissertation, University of Florida, Gainesville, FL
15. Gutiérrez-E MA, Luth D, Moore GA (1997) Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus. Plant Cell Rep 16: 745–753CrossRef
16. Hidaka T, Omura M, Ugaki M, Tomiyama M, Kato A, Ohshima M, Motoyoshi F (1990) Agrobacterium-mediated transformation and regeneration of Citrus spp. from suspension cells. Jpn J Breed 40: 199–207
17. Hidaka T, Omura M (1993) Transformation of citrus protoplasts by electroporation. J Japan Soc Hort Sci 62: 371–376CrossRef
18. Hodgson RW (1967) Horticultural varieties of citrus. In: Reuther W, Batchelor LD, Webber HJ (eds) The citrus industry, Vol 1. University of California Press, Berkeley, pp 431–591
19. Hood EE, Helmer GL, Fraley RT, Chilton M-D (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol 168: 1291–1301PubMed
20. Horowitz RM, Gentili B (1969) Taste and structure in phenolic glycosides. J Agr Food Chem 17: 696–700CrossRef
21. Horowitz RM, Gentili B (1977) Flavonoid constituents of Citrus. In: Nagy S, Shaw PE, Veldhuis MK (eds) Citrus Science and Technology, AVI Publishing, Westport, CT, pp 397–426
22. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387–405CrossRef
23. Kaneyoshi (Hiramatsu) J, Kobayashi S, Nakamura Y, Shigemoto N, Doi Y (1994) A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.) Plant Cell Rep 13: 541–545
24. Kobayashi S, Uchimiya H (1989) Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer. Jpn J Genet 64: 91–97CrossRef
25. Kobayashi S, Nakamura Y, Kaneyoshi J, Higo H, Higo K (1996) Transformation of kiwifruit (Actinidia chinensis) and trifoliate orange (Poncirus trifoliata) with a synthetic gene encoding the human epidermal growth factor ( HEGF ). J Jpn Hort Sci 64: 763–769
26. Litz RE, Moore GA, Srinivasan C (1985) In vitro systems for propagation and improvement of tropical fruits and palms. Hort Rev 7, AVI Publishing Co., Westport CT, 43 pp
27. Luth D (1997) Transgenic approaches to modulating naringin content in Citrus paradisi. Ph.D. dissertation, University of Florida, Gainesville.
28. Moore GA. 1986. In vitro production of Citrus rootstocks. HortSci. 21: 300–301
29. Moore GA, Castle WS (1988) Morphological and isozymic analysis of open-pollinated Citrus rootstock populations. J Hered 79: 59–63
30. Moore GA, Jacono CC, Neidigh JL, Lawrence SD, Cline K (1992) Agrobacterium-mediated transformation of Citrusstem segments and regeneration of transgenic plants. Plant Cell Rep 11: 238–242
31. Moore GA, Jacono CC, Neidigh JL, Lawrence SD, Cline K (1993) Transformation in Citrus. In: YPS Bajaj (ed.) Biotechnology in Agriculture and Forestry, Vol 23. Springer-Verlag, Berlin Heidelberg New York. pp 194–208
32. Pena L, Cervera M, Juarez J, Navarro A, Pina JA, Duran-Vila N, Navarro L (1995a) Agrobacterium-mediatedtransformation of sweet orange and regeneration of transgenic plants. Plant Cell Rep 14: 616–619
33. Pena L, Cervera M, Juarez J, Ortega C, Pina JA, Duran-Vila N, Navarro L (1995b) High-efficiency Agrobacterium-mediated transformation and regeneration of citrus. Plant Sci 104: 183–191CrossRef
34. Pena L, Cervera M, Juarez J, Navarro A, Pina JA, Navarro L (1997) Genetic transformation of lime (Citrus aurantifolia Swing.): Factors affecting transformation and regeneration. Plant Cell Rep 16: 731–737
35. Perez-Molphe-Balch E, Ochoa-Alejo N (1998) Regeneration of transgenic plants of Mexican lime from Agrobacterium rhizogenes transformed tissues. Plant Cell Rep 17: 591–596CrossRef
36. Rouseff RL, Martin SF, Youtsey CO (1987) Quantitative survey of narirutin, naringin, hesperdin, and neohesperidin in Citrus. J Agric Food Chem 35: 1027–1030CrossRef
37. Scora RW (1975) On the history and origin of citrus. Bull Torrey Bot Club 102: 369–375CrossRef
38. Sekiya ME, Lawrence SD, McCaffery M, Cline K (1991) Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus. J Gen Virol 72: 1013–1020PubMedCrossRef
39. Slightom JL (1991) Custom polymerase-chain-reaction engineering of a plant expression vector. Gene 100: 251–255PubMedCrossRef
40. Soost RK, Cameron JW (1975) Citrus. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University, West Lafayette, Indiana, pp 507–540
41. Stewart I (1977) Citrus color–a review. Proc Int Soc Citriculture 1: 308–311
42. Vardi A, Bleichman S, Aviv D (1990) Genetic transformation of Citrus protoplasts and regeneration of transgenic plants. Plant Sci 69: 199–206CrossRef
43. Yao J-L, Wu J-H, Gleave AP, Morris BAM (1996) Transformation of citrus embryogenic cells using particle bombardment and production of transgenic embryos. Plant Science 113: 175–183CrossRef

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