Olive (Olea europaea var. sativa) Transformation

Published Date
Volume 66 of the series Forestry Sciences pp 245-279

Author 

• Rugini Eddo
• , Biasi Rita
• , Muleo Rosario

Abstract

The olive tree grows typically in the Mediterranean Basin. It probably originated in Asia Minor and was put under cultivation in countries of the seacoast of the eastern Mediterranean between 1200–1700 B. C., depending on the regions. In the beginning, olive was more likely to be cultivated to be eaten as fruit, which contains 15 to 25% fat and was easy to be stored. Olives therefore constituted an important component of the human diet.

References

1. Acebedo, M.M., Lavee, S., Linan J., and Troncoso, A. (1997) Germination of embryos for speeding up seedling development in olive breeding programmes. Sci. Hort. 69, 207–215.
2. Altamura Betti, M..M., Altamura, L. and Mazzolani, G. (1985) Elements for the revision of the genus Olea (Tourn.) Linn. VI. The taxa present in Oceania which can be ascribed to Olea and allied genera. Ann. Bot. XLIII, 45–52.
3. Altamura, L., Altamura Betti, M.M., and Mazzolani, G. (1987) Elements for the revision of the genus Olea (Tourn.) Linn. VII. The taxa present in Asia which can be ascribed to Olea and allied genera. Ann. Bot. XLV, 119–134.
4. Angeles Revilla, M., Pacheco, J., Casares, A., and Rodriguez, R. (1996) In vitro reinvigoration of mature olive trees (Olea europaea L.) through micrografting. In vitro Cell Dev. Biol.-Plant 32, 257–261.
5. Angiolillo, A., Mencuccini, M., and Baldoni, L. (1999) Olive genetic diversity assessed using amplified fragment lenght polymorphisms. Theor Appl Genet. 98, 411–421.
6. Asturias, J.A., Arilla, M.C., Gomez-Bayon, N., Martinez, J., Martinez, A., Palacios, R. (1997) Cloning and expression of the panallergen profilin and the major allergen (Ole e I) from olive tree pollen. J. Allergy Clin Immunol. 100, 365–372.PubMed
7. Baldoni, L., Georgi, L.L., and Abbott, A.G. (1996) Nucleotide sequence of a cDNA clone from Olea europaea encoding a stearoyl acyl carrier protein desaturase (Accession No. U58141) (PGR96–052). Plant Physiol. 111, 1353.
8. Bao Z-H, Ma Y-F, Liu J-F, Wang K-J, Zhang P-F, Ni D-X, Yang W-Q (1980) Induction of plantets from the hypocotyl of Olea europaea L. in vitro. Acta Bot Sin 2, 96–97 (in Chinese)
9. Baraldi, R., Cristoferi, G., Facini, 0., and Lercari B. (1992) The effect of light quality in Prunus cerasus. I. Photoreceptors involved in internode elongation and leaf expansion in juvenile plants. Photochem and Photobil 56, 541–544.
10. Bartolini, S., and Guerriero, R. (1995) Self-compatibility in several clones of oil olive cv. Leccino. Adv. Hort. Sci. 9, 71–74.
11. Batanero, E., Ledesma, A., Villalba, M., and Rodriguez, R., (1997) Purification, amino acid sequence and immunological characterization of Ole e 6, a cysteine-enriched allergen from olive tree pollen. FEBS Lett 410, 93–296.
12. Bellini, E. (1990) Behaviour of some genetical characters in olive seedlings obtained by crossbreeding. Proceedings XXIII International Horticultural Congress, Florence. Abstracts of contributed papers. 2. Poster, 3062.
13. Bellini, E. (1993) Genetic variabiltiy and heritability of some characters in cross-bred olive seedlings. Olivae 49, 2 1–34.
14. Bellini, E., Pariah, M.V., Pandolfi, S., Giordani, E., Perri, E., and Silletti. A. (1995) Miglioramento genetico dell’ olivo: prime osservazioni su selezioni ottenute da incrocio. Italus Hortus 2 (1–2), 3–12.
15. Bellini, E., Pariah. M.V., Pandolfi, S., Giordani, E., Perri, E. and Silletti. A., 1995. Miglioramento genetico deli’ olivo: prime osservazioni Su selezioni ottenute da incrocio. Italus Hortus 2, 3–12
16. Berenguer, A. G. (1978) Seleccion clonal en Olivo (Olea europaea L.). Olea 6, 7–15.
17. Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acid Res. 12, 8711–8721.PubMed
18. Bitonti, M.B., Cozza, R., Chiappetta, A., Contento, A., Minelli, S., Ceccarelli, M., Gelati, M.T., Maggini, F., Baldoni, L., and Cionini, P.G. (1999) Amount and organizationn of the heterochromatin in Olea europaea and related species. Heredity (in press)
19. Bogani, P., Cavalieri, D., Petruccelli, R., Polsinelli, L., and Roselli, G. (1994) Identification of olive tree cultivars by using random amplified polymorphic DNA. Acta Hort. 356, 98–101.
20. Bogani, P., Mugnai, M.A., Simoni, A., Lio, P., and Buiatti, M. 1999. Characterization of differences in RAPD patterns from juvenile and adult olea leaves (submitted)
21. Bongi g. and Long S. (1987) Light-dependent damage to photosynthesis in olive leaves during chilling and high temperature stress. Plant Cell Environ, 10, 241–249
22. Briccoli Bati C. Fodale A. Mulè R. and Trombino T. (1999). Trials to increase in vitro rooting of Olea europaea L. cuttings. Acta Hortic., 474, 91–94
23. Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S., Mauvais, C.J. and Broglie, R. (1991) Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254, 1194–1197.
24. Canas, L.A, and Benbadis, A. (1988) Plant regeneration from cotyledon fragments of the olive tree (Olea europaeaL.). Plant Sci. 54, 65–74.
25. Canas, L.A, Wyssmann, A.M., Benbadis, M.C. (1987) Isolation, culture and division of olive (Olea europaea L) protoplasts. Plant Cell Rep 5, 369–371.
26. Cardarelli,M., Mariotti, D., Pomponi, M., Spanò, L., Capone, I., and Costantino, P. (1987) Agrobacterium rhizogenesT-DNA genes capable of inducing hairy root phenotype. Mol. Gen. Genet. 209, 475–480.
27. Carmona, J.J., Molina, A., Fernandez, J.A., Lopez-Fando, J.J., and Garcia-Olmedo, F. (1993) Expression of the a-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. Plant J. 3, 457–462.PubMed
28. Cavalioeri, D., Petruccelli, R., Polsinelli, L., and Roselli, G. (1998) Studio della variabilità fra cloni e fra cultivar in Olea europaea L. IV Congresso Nazionale Biodiversità germoplasma locale e sua utilizzazione. Alghero 8–11 settembre 1998.
29. Charest, P.J., Caléro, N., Lachance, D., Datla, R.S.S., Duchesne, L.C., and Tsang, E.W.T. (1993) Microprojectile-DNA delivery in conifer species. Plant Cell Rep. 12, 189–193.
30. Chevalier, A. (1948) L’origine de l’Olivier cultiv, et ses variations. Rev. Bot. Appl. 303–304, 1–25.
31. Chilton, M.D., Tepfer, D.A., Petit, A., Casse-Delbart, F., and Tempe, J. (1982) Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295, 43 2434.
32. Columella (1977) Liber quintus. In: L’arte dell’Agricoltura (Libri rei rusticae), G.Einaudi, Ed., pp 379–389. Torino, Italy.
33. Cresti, M., Linskens, H.F., Mulcahy, D.L., Bush, S., Di Stilio, V., Xu, M.Y., Vignani, R., and Cimato A. (1996) Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea. Adv. Hort. Sci. 10, 105–107.
34. Cronquist A. (1981). An integrated system of classification of flowering plants. Columbia Univ. Press, NY
35. Dandekar, A., McGranahan, G.H., and James, D.J. (1993) Transgenic woody plants, in: Transgenic Plants, 2. Academic Press, London, pp. 129–151.
36. Dandekar, A., McGranahan, G.H., and James, D.J. (1993) Transgenic woody plants, in: Transgenic Plants, 2. Academic Press, London, pp. 129–151.
37. Delrio, G. (1995) Controllo integrato dei fitofagi dell’olivo. Inform. Fitopatologico 12, 9–15.
38. Duchesne, L.C., Lelu, M.A., von Aderkas, P., and Charest, P.J. (1993) Microprojectilemediated DNA delivery in haploid and diploid embryogenic cells of Larix spp. Can J For Res 23, 312–316.
39. Ellis, D.D., McCabe, D.E., Russel, D., Martinell, B., and McCown, B.H. (1991) Expression of inducible angiosperm promoters in a gymnosperm, Picea glauca (white spruce). Plant Mol Biol 17, 19–27.PubMed
40. Fabbri, A., Hormaza, J.J., and Polito V.S. (1995) Random amplified polymorphic DNA analysis of olive (Olea europaea L.) cultivars. J. Amer. Soc. Hort. Sci. 120, 538–542. Fedeli, E. (1983) Les composants mineurs des lipides. RFCG, 30, 51–60.
41. Fontanazza, G. (1987) Presentiamo la cultivar 1–77“. Terra e Vita 46, 10–1 1.
42. Fontanazza, U., Baldoni, L., and Corona, C. (1990) Osservazioni preliminari sul valor agronomico di una nuova cultivar da olio: “Fs-17”. Proceedings Problematiche qualitative dell’olio d’oliva. Accademia Nazionale dell ‘Olivo–Spoleto, 69–75.
43. Fontanazza. G., (1993). Olivicoltura intensiva meccanizzata. Edagricole. Bologna. Goldfarb, B., Strauss, S.H., Howe, G.T., and Zaerr, J.B. (1991) Transient gene expression of microprojectile-introduced DNA in Douglas-fir cotyledons. Plant Cell Rep 10, 517–521.
44. Fontanazza, G. (1993) Olivicoltura intensiva meccanizzata. Edagricole. Bologna.
45. Fontanazza, G. and Bartolozzi F. (1998). Olive, In: Italian contribution to plant genetics and breeding. Scarascia-Mugnozza GT and Pagnotta M. A. ( eds ), EUCARPIA, pp 723–748
46. Fontanazza, U., Baldoni, L. and Corona, C., 1990. Osservazioni preliminari sul valor agronomico di una nuova cultivar da olio: “Fs-17”. Proceedings Problematiche qualitative dell’olio d’oliva. Accademia Nazionale dell’Olivo, Spoleto, pp. 69–75.
47. Fontanazza, U., Baldoni, L., and Corona, C. (1990) Osservazioni preliminari sul valor agronomico di una nuova cultivar da olio: “Fs-17”. Proceedings Problematiche qualitative dell’olio d’oliva. Accademia Nazionale dell ‘Olivo–Spoleto, 69–75.
48. Fontanazza. G., (1993). Olivicoltura intensiva meccanizzata. Edagricole. Bologna. Goldfarb, B., Strauss, S.H., Howe, G.T., and Zaerr, J.B. (1991) Transient gene expression of microprojectile-introduced DNA in Douglas-fir cotyledons. Plant Cell Rep 10, 517–521.
49. Gonzalez-Rio, F., Gurriaran, M.J., Gonzalez-Benito E., and Revilla, M.A. (1994) Dessication and cryopreservation of olive (Olea europaea L.) embryos. Cryo-Letters 15, 337–342.
50. Green, P.S., and Wickens, G.E. (1989) The Olea europaea complex. In: Kit Tan (Ed.), The Davis and Hedge Festeschrift Edinburg University Press, Edinburgh, pp. 287–299
51. Gucci, R. (1998) Assimilazione e ripartizione del carbonio in foglie d’olivo. Rivista di Frutticoltura 7/8, 77–82.
52. Gutierrez-Pesce, P., Taylor, K., Muleo, R., and Rugini, E. (1998). Somatic embryogenesis and shoot regeneration from transgenic roots of the cherry rootstock “Colt” (Prunus avium x P. pseudocerasus) mediated by pRi 1855 T-DNA of Agrobacterium rhizogenes. Plant Cell Rep. 17, 574–580.
53. Hain, R., Reif, H.J., Krause, E., Langebartels, R., Kindl, H., Vornam, B., Wiese, W., Schmelzer, E., Schreicher, P.H., Stocker, R.H., and Stenzel, K. (1993) Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361, 153–156.PubMed
54. Hammerschlag, F.A. and Smigocki, A.C. (1998) Growth and in vitro propagation of peach plants transformed with the shooty mutant strain of Agrobacterium tumefaciens. HortSci. 33, 897–899.
55. Hartman HT, Schnathorst WC and Whisler, J (1971) “Oblonga” a clonal olive rootstock resistant to Verticillium vilt. Calif. Agricult. 25:12
56. Hartmann,H.T., and Loreti, F. (1965) Seasonal variation in the rooting of olive cuttings. Proc Am Soc Hort Sci 87, 194–198.
57. Hartmann, H.T., and Porlingis, I. (1957) Effect of different amount of winter chilling on fruitfulness of several olive varieties. Bot Gaz 119, 102–104.
58. Hay, I., Lachance, D., von Aderkas, P., and Charest, P.J. (1994) Transient chimeric gene expression in pollen of five conifer species following microparticle bombardment. Can J For Res 24, 2417–2423.
59. Hightower, R., Baden, C., Penzes, E., Lund, P., and Dunsmuir, P. (1991) Expression of antifreeze proteins in transgenic plants. Plant Mol. Biol. 17, 1013–1021.PubMed
60. Huang, Y., Nordeen, R.O., Di, M., Owens, L.D., and McBeath, J.H. (1997) Expression of an engineered cecropin gene cassette in transgenic tobacco plants confers disease resistance to Pseudomonas syringae pv. tabaci. Mol. Plant Pathol. 87, 494–499.
61. Huttzinger, O. Kosuge, T. (1968). 3-indole-acetyl —L-lysine, a coniugate of 3-indolacetic acid produced by Pseudomonas savastanoi. Biochemistry, 7, 183–194.
62. Inserra, R.N., Vovlas, N., Fontanazza, U., and Casta, G. (1981) Comportamento di alcune cultivar di olivo alle infestazioni di quattro specie di nematodi. Riv. Orto . lorofrutt. It. 65 (2), 143–148.
63. Jaglo-Ottosen, K.R., Gilmour, S.J., Zarka, D.G., Schabenberger, O., and Thomashow, M.F. (1993) Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science 280, 104–106.
64. Katsiotis, A., Hagidimitriou, M., Douka, A., and Hatzapoulos, P. (1998) Genomic organization, sequence interrationship, and physical localization using in situ hybridization of two tandemly repeated DNA sequences in the genus Olea. Genome 41, 527–534.
65. Katsiotis, A., Hagidimitriou, M., Douka, A., and Hatzopoulus P. (1998). Genomic organization, sequence interrelationship, and physical localization using in situ hybridization of two tandemly repeat DNA sequences in the genus Olea. Genome 41, 527534.
66. Lambardero, M., Barbas, J.A., Moscoso del Prado, J., and Carreira, J. (1994) cDNA sequence analysis of the main olive allergen, Ole e I. Clin Exp Allergy 24, 765–770.
67. Lambardi M, A. Caccavale, G. Caricato and E. Rugini histological observations on somatic embryos of olive (Olea europaea L.). Acta Hortic, 474: 505–509
68. Lambardi, M., Capuana, M., Sozzi, L., and Giannini, R. (1995) Factors affecting in vitro adventitious bud induction from excised embryos of Swiss stone pine (Pinus cembra L.). For. Genet. 2, 49–58.
69. Ledesma, A., Villalba, M., Batanero, E., and Rodriguez, R. (1998) Molecular cloning and expression of active Ole e 3, a major allergen from olive-tree pollen and member of a novel family of Ca2+ binding proteins (polcalcins) involved in allergy. Eur J. Biochem 258, 454–459PubMed
70. Leva, A.R., Maleo, R., and Petruccelli, R. (1995) Long-term somatic embryogenesis from immature olive cotyledons. J. Hortic. Sci. 70, 10–15.
71. Leva, A.R., Petruccelli, U., and Bartolini, U. (1994) Mannirol `in vitro’ culture of Olera europaea L. (cv. Maurino). Acta Hort 356, 43–46.
72. Ledesma, A., Villalba, M., Batanero, E., and Rodriguez, R. (1998) Molecular cloning and expression of active Ole e 3, a major allergen from olive-tree pollen and member of a novel family of Ca2+ binding proteins (polcalcins) involved in allergy. Eur J. Biochem 258, 454–459PubMed
73. Longemann, J., Jach, G., Tommerup, H., Mundy, J., and Schell, J. (1992) Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants. Bio/Technology 10, 305–308.
74. Marte, M., Gadani, V., Savino, V. and Rugini, E. (1985). Strawberry latent ringspot virus associated with a new desease of olive in Central Italy. Plant Desease, 70:171–172.
75. Martelli G.P. 1998. Enfermedades infecciosas y certificacion del olivo: panorama general. Phytoma Espana, 102, 180–186.
76. Martelli G.P. 1999. Infectious diseases and certification of olive: an overview. EPPO Bulletin,in press.
77. Martelli G.P., Prota U. 1997. Malattie e patogeni dell’olivo trasmessi col materiale di propagazione. I Georgofili, Quaderni II, 73–96.
78. Martelli G.P., V. Savino, B. Di Terlizzi, L. Catalano and S. Sabanadzovic. 1995. Viruses and certification of olive in Apulia. Acta Horticolturae 386, 569–573.
79. Martsinkovskaya, A.I., Poghosyan Z.P., Haralampidis, K., Murphy, D.J., and Hatzopoulus, P. (1999). Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives. Plant Mol Biol. 40, 9–90.
80. Mazzolani, G., and Altamura Betti, M.M. (1981) Elementi per la revisione del genere Olea (Tourn.) Linn. V-Olea copensis L.: forme affini. Ann. Bot. XXXIX (2), 177–196.
81. Mazzolani, G., and Altamura Betti, M.M. (1976–77) Elementi per la revisione del genere Olea (Tourn) Linn. 1 -Nota introduttiva. Annali di Botanica Roma, 35–36, 463–469.
82. Mazzolani, G., and Altamura Betti, M.M. (1979) Elementi per la revisione del genere Olea (Tourn). Linn. III -Olea Crysophylla Lam: forme affini. Ann Bot 28, 15–31.
83. Mazzolani, G., and Altamura Betti, M.M. (1980) Elementi per la revisione del genere Olea (Tourn) Linn. IV: Ciclo di Olea capensis L. Ann Bot 28, 149–172.
84. McKersie, B.D., Chen, Y., de Beus, M., Bowley, S.R., Bowler, C., Inzé, D., D’Halluin, K., and Botterman, J. (1993) Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L). Plant Physiol. 103, 1155–1163.PubMed
85. Mekuria, G.T., Collins, G.G., and Sedgley, M. (1999) Genetic variability between different accessions of some common commercial olive cultivars. J. Hort. Sci and Biotech. 74, 309314.
86. Mencuccini M (1994) Agrobacterium-mediated gene transfer studies for genetic transformation of olive (Olea europaea L. tissues. Proc. VIIIth Int. Cong. of Plant Tissue and Cell Culture. Florence 12–17 June, p 67.
87. Mencuccini M. Micheli M. Angiolillo A. and Baldoni L. (199) Genetic transformation of olive (Olea europaea L.) using Agrobacterium tumefaciens Acta Hortic. 474, 515–519.
88. Mencuccini M. Micheli M. Angiolillo A. and Baldoni L. (1997). Trasferimento genico in olivo mediante Agrobacterium tumefaciens. Proc. XLI SIGA p. 123
89. Mencuccini, M., and Rugini E. (1993) In vitro shoot regeneration from olive cultivars tissues. Plant Cell Tissue and Organ Culture 32, 283–288.
90. Mencuccini, M.. (1991) Protoplast culture isolated from different tissues of olive (Olea europoea L.) cultivars. Physiol. Plant. 82, A14.
91. Micheli, M., Mencuccini, M., and Standardi, A. (1998) Encapsulation of in vitro proliferated buds of olive. Adv. Hort. Sci. 12, 163–168.
92. Mills, D., Hammerschlag, F.A., Nordeen, R.O., and Owens, L.D. (1994) Evidence for the breakdown of cecropin B by proteinases in the intercellular fluid of peach leaves. Plant Sci. 104, 17–22.
93. Mitrakos, K., Alexaki, A., and Papadimitriou, P. (1992) Dependance of olive morphogenesis on callus origin and age. J. Plant Physiol. 139, 269–273.
94. Morettini, A. (1972) Olivicoltura. Reda, Roma, pp. 522.
95. Mule, R., Fodale, A.S., Parlati, M..V., and Tucci, A. (1992) Primi risultati sulla coltura in vitro di antere di olivo (Olea europaea L.). Agricoltura Ricerca 140, 69–72.
96. Muleo, R. and Thomas, B. (1993) The role of light quality in the control of branching in Prunus cerasifera shoot tip cultured in vitro. European Symp. Photomorphogenesis in Plants, Abstract.
97. Muleo, R. and Thomas, B. (1997) Effect of light quality on shoot proliferation of Prunus cerasifera in vitro are the result of differential effects on bud induction and apical dominance. J. Hort. Sci. 72, 483–491.
98. Nakajima, H., Muranaka, T., Ishige, F., and Akutsu, K. (1997) Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme. Plant Cell Rep. 16, 674–679.
99. Norelli, J.L., Aldwinckle, H.S., Destefano-Beltran, L., and Jaynes, J.M. (1994) Transgenic `Mailing 26’ apple expressing the attacin E gene has increased resistance to Erwinia amylovora. Euphytica 77, 123–128.
100. Ochman, H., Medhora, M.M., Garza, D., and Hartl, D.L. (1990) Amplification of flanking sequences by inverse PCR, in: Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (eds). PCR Protocols, Academic Press, San Diego pp. 219–227.
101. Orinos, T., and Mitrakos, K. (1991) Rhizogenesis and somatic embryogenesis in calli from wild olive [Olea europaeavar sylvestris (Miller) Lehr] mature zygotic embryos. Plant Cell Tissue and Organ Culture 27, 183–187.
102. Ouazzani, N., Lumaret, R., Villemur, P., and Di Giusto, F. (1993). Leaf allozye variation in cultivated and wild olive trees (Olea europaea L.) J Hered. 84, 34–42.
103. Owens, L.D. (1995) Overview of gene availability, identification, and regulation. HortSci. 30, 957–961.
104. Ochman, H., Medhora, M.M., Garza, D., and Hartl, D.L. (1990) Amplification of flanking sequences by inverse PCR, in: Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (eds). PCR Protocols, Academic Press, San Diego pp. 219–227.
105. Orinos, T., and Mitrakos, K. (1991) Rhizogenesis and somatic embryogenesis in calli from wild olive [Olea europaeavar sylvestris (Miller) Lehr] mature zygotic embryos. Plant Cell Tissue and Organ Culture 27, 183–187.
106. Pannelli, U., Famiani, F., Rugini, E., Bignami, D., and Natali, S. (1990) Preliminary characteristics of olive somatic mutants from gamma irradiated `Frantoio’ and `Leccino’ piantlets. Acta Hort 286, 77–80.
107. Parlati, M.V., Bellini, E., Perri, E., Pandolfi, S., Giordani, E. and Martelli, (1994). Genetic improvement of olive: initial observations on selections made in Florence. Ada Her 356: and7–90.
108. Parlati, M.V., Bellini, E., Perri, E., Pandolfi, S., Giordani, E., and Martelli, S. (1994) Genetic improvement of olive: initial observations on selections made in Florence. Acta Hort. 356, 87–90.
109. Perri, E., Lombardo, N., Godino G., Sirianni,R., and Tartarini, S. (1997) Caratterizzazione di cultivar di olivo (Olea europaea L.) italiane mediante marcatori RAPD. Agro-Bio-Frut: Cesena 9 maggio 1997. pp. 19–20.
110. Perri, E., Parlati, M.V., and Rugini, E. (1994) Isolation and culture of olive (Olea europaea L.) cultivar protoplasts. Acta Hort 356, 5 1–53.
111. Perri, E., Parlati, M.V., Mulé, R.,and Fodale, A.S. (1994) Attempts to generate haploid plants from in vitro cultures of Olea europaea L. anthers. Acta Hort 356, 47–50.
112. Pontikis, C.A., Loukas, M., and Kousonis, G. (1980) The use of biochemical markers to distinguish olive cultivars. J. Hort. Sci 55, 333–343.
113. Porlingis, I.C., and Therios, I. (1976) Rooting response of juvenile and adult leaf olive cuttings to various factors. J. Hort. Sci. 22, 31–39.
114. Prota, U. (1995) Le malattie dell’olivo. Informatore Fitopatologico 12, 16–26.
115. Roselli, G. (1972) Mutazioni spontanee e piante chimeriche nell’olivo. Genetica Agraria 26 (1–2), 62–74.
116. Roselli, G., and Scaramuzzi, F. (1974) Definizione dei termini di cultivar e clone. Atti Accad Agr Bologna 2, 119.
117. Roselli, G., Petruccelli, R., Bogani, P., and Polsinelli, L. (1995) Variabilità inter e intravarietale in olivo determinata mediante tecnica RAPD. Agrobiotec 95: Biotecnologie avanzate e agricoltura. Ferrara 19–21 ottobre 1995.
118. Roselli, U., and Donini, B. (1982) “Briscola”. Nuova cultivar di olivo a sviluppo compatto. Riv. Onoflorofrutt. It. 66 (2), 103–114.
119. Rotino, G.L., Peni, E., Zottini, M., Sommer, H., and Spena, A. (1997) Genetic engineering of parthenocarpic plants. Nature biotech. 15, 1398–1401.
120. Rugini, E. (1984) In vitro propagation of some olive (Olea europaea L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Sci Hortic 24, 123–134.
121. Rugini E, Di Francesco G, Muganu M, Astolfi S and Caricato G. (1997) The Role of Polyamines and hydrogen peroxide in root formation in olive cuttings and in vitro explants. In: Biology of Root Formation and Development, by Altman and Waisel eds, pp 65–74, chap. 8 Plenum Publishing Corporation New York
122. Rugini E., Gutierrez-Pesce P., Spampinato P.L., Ciarmiello A., D’Ambrosio C. (1998) New perspective for Biotechnologies in olive breeding: morphogenesis, in vitro selection and gene transformation. Acta Hortic, 474:107–110
123. Rugini, E., Pannelli, G., Ceccarelli, M,. and Muganu,M. (1996) Isolation of triploid and tetraploid olive (Olea europaea L.) plants from mixoploid cv Frantoio and Leccino mutants by in vivo and in vitro selection. Plant Breeding 115, 23–27.
124. Rugini E, Di Francesco G, Muganu M, Astolfi S and Caricato G. (1997) The Role of Polyamines and hydrogen peroxide in root formation in olive cuttings and in vitro explants. In: Biology of Root Formation and Development, by Altman and Waisel eds, pp 65–74, chap. 8 Plenum Publishing Corporation New York
125. Rugini, E. (1986) Olive, in: Bajaj, Y.P.S. (ed) Biotecnology in Agriculture and Forestry Vol 10. Springer-Verlag. Berlin Heidelberg, New York. pp. 253–267
126. Rugini, E. (1988) Somatic embryogenesis and plant regeneration in Olive (Olea europaea L.). Plant Cell, Tissue and Organ Culture 14, 207–214.
127. Rugini, E. (1992). Involvement of polyamines in auxin and Agrobacterium rhizogenes-induced rooting of fruit trees in vitro. J. Amer. Hort. Sci. 117: 532–536.
128. Rugini, E. (1995). Somatic embryogenesis in olive (Olea europaea L.). In: Somatic embryogenesis in woody plants, Vol II ( S.M. Jain, P.k. Gupta and R.J. Newton eds ), Kluwer Academic Publishers p. 171–189
129. Rugini, E. and Gutierrez-Pesce, P. (1999) Transformation in Prunus species, in: Bajaj, Y.P.S. (ed) Biotecnology in Agriculture and Forestry 44, Springer-Verlag, Berlin, Heidelberg, New York, London, Paris, Tokio, pp. 245–262.
130. Rugini, E. and Mariotti, D. (1992) Agrobacterium rhizogenes T-DNA genes and rooting in woody species. Acta Hort.300, 301–308.
131. Rugini, E. and Tarini, P. (1986) Somatic embryogenesis in olive (Olea europaea L.). In: MoetHennessy (Ed.), Proceedings Conference Fruit Tree Biotechnology - Paris (France). p. 62.
132. Rugini, E., and Caricato, G. (1995) Somatic embryogenesis and plant recovery from mature tissues of olive cultivars (Olea europaea L.) “Canino” and “Moraiolo”. Plant Cell Rep. 14, 257–260.
133. Rugini, E., and Lavee, S. (1992) Olive Biotechnology. in: Hammerschlag, F.A. and Litz, R. eds Biotechnology of Perennial Fruit Crops. Hardwick T. Book Publisher pp 371–382.
134. Rugini, E., and Muganu, M. (1998) A novel strategy for the induction and maintenance of shoot regeneration from callus deri ved from established shoots of apple [Malus x domestica BorkH.] cv. golden delicious. Plant Cell Reports 17, 581–585
135. Rugini, E., Caricato, G., Muganu, M., Taratufolo, C., Camili, M., and Cammilli, C. (1997) Genetic stability and agronomic evaluation of six-year-old transgenic kiwi plants for rol ABC and rol B genes. Acta Hort. 447, 609–610.
136. Rugini, E., Muganu, M., Pilotti, M., Balestra, G.M., Varvaro, L., Magro, P., Bressan, R., and Taratufolo, C. (1999) Genetic Stability, transgene hereditability and agronomic evaluation of transgenic kiwi (Actinidia deliciosa A. Chev.) Plants for rol ABC, rol B and Osmotin genes. Forth International Symposium on Kiwifruit Santiago del Chile 11–14 Febr. p 26
137. Rugini, E., Pellegrineschi, A., Mencuccini, M., and Mariotti D. (1991) Increase of rooting ability in the woody species kiwi (Actinidia deliciosa A. Chev.) by transformation with Agrobacterium rhizogenes rol genes. Plant Cell Rep. 10, 291–295
138. Rugini, E., Politi, V., Bignami, C., De Agazio, M., and Grego, S. (1991). Effect of polyamine treatment on rooting of three olive cultivar cutting. Acta Hortic. 286, 97–100
139. Rugini, E., Muganu, M., Pilotti, M., Balestra, G.M., Varvaro, L., Magro, P., Bressan, R., and Taratufolo, C. (1999) Genetic Stability, transgene hereditability and agronomic evaluation of transgenic kiwi (Actinidia deliciosa A. Chev.) Plants for rol ABC, rol B and Osmotin genes. Forth International Symposium on Kiwifruit Santiago del Chile 11–14 Febr. p 26
140. Rugini, E., Pellegrineschi, A., Mencuccini, M., and Mariotti D. (1991) Increase of rooting ability in the woody species kiwi (Actinidia deliciosa A. Chev.) by transformation with Agrobacterium rhizogenes rol genes. Plant Cell Rep. 10, 291–295
141. Saiki, R.K. (1990) Amplification of genomic DNA, in: Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (eds) PCR Protocols. Academic Press, San Diego pp. 13–20.
142. Scaramuzzi,F, and Roselli, G. (1986) Olive genetic improvement. Olea 17, 7–17.
143. Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. of Mol. Biol. 98, 503–517.
144. Tarczynski, M.C. Jensen, R.G., and Bohnert, H.J. (1993) Stress protection of transgenic tobacco by production of the osmolyte mannitol. Science 259, 508–510.
145. Tattini, M. (1993) Il ciclo di produzione delle piante di olivo in vivaio. Rivista di Frutticoltura 1, 17–24.
146. Taylor, H. (1945) Cyto-taxonomy and phylogeny of the Oleaceae. Brittonia 5, 337–339. Tombesi, A. (1978) La fertilità nell’olivo. Riv. Ortoflorofrutt. It. 62 (4), 435–450.
147. Tombesi, A. (1994) Olive fruit growth and metabolism. 2nd Int. Symp. On Olive Growing. Jerusalem, Israel, 1993 Acta Hortic 356,. 225–232.
148. Tombesi, A. (1995) Fisiologia dell’olivo, tecniche colturali e loro interazioni. Rivista di Frutticoltura 12, 15–21.
149. Trujillo, I., Rallo, L., Carbonell E.A., and Asins M.J. (1990) Isoenzymatic variability of olive cultivars according to their origin. Acta Hort. 286, 137–140.
150. Tucker, K. J. (1976) Effect of far-red light of the hormonal control of side shoot growth in the tomato. Ann. Bot. 40, 1033–1042.
151. Vaeck, M., Raynaerts, A., Hofte, H., Jansens, S., DeBeukeleer, M., Dean, C., Zabeau, M., van Montagu, M., and Leemans, J. (1987) Transgenic plants protected from insect attack. Nature 328, 33–37.
152. Van Camp, W., Willekens, H., Bowler, C., Van Montagu, M., Inze, D., Reupold-Popp, P., Sandermann, H., and Langebartels, C. (1994) Elevated levels of superoxide dismutase protect transgenic plants against ozone damage. Bio/Technology 12, 165–168.
153. Vergari, G., Patumi, M., and Fontanazza, G. (1996) Utilizzo dei marcatori RAPDs nella caratterizzazione del germoplasma di olivo. Olivae 60, 19–22.
154. Villalba, M., Batanero, E., Monsalve, R.I., Gonzalez de la Pena, M.A., Lahoz, C., and Rodriguez, R. (1994). Cloning and expression of Ole e I, the major allergen from olive tree pollen. Polymorphism analysis and tissue specificity. J. Biolo Chem 269, 15217–15222.
155. Vince-Prue, D. and Canham, A. E. (1983). Horticultural significance of photomorphogenesis, in: Shropshire and Mohr (ed). Photomorphogenesis. Encyclopedia of Plant Physiology. Springer Verlag. pp. 518–544.
156. Vaeck, M., Raynaerts, A., Hofte, H., Jansens, S., DeBeukeleer, M., Dean, C., Zabeau, M., van Montagu, M., and Leemans, J. (1987) Transgenic plants protected from insect attack. Nature 328, 33–37.
157. Van Camp, W., Willekens, H., Bowler, C., Van Montagu, M., Inze, D., Reupold-Popp, P., Sandermann, H., and Langebartels, C. (1994) Elevated levels of superoxide dismutase protect transgenic plants against ozone damage. Bio/Technology 12, 165–168.
158. Wiesma, Z., Avidan, N., Lavee, S., and Quebedeaux B. (1998) Molecular characterization of common olive varieties in Israel and the west bank using randomly amplified polymorphic DNA (RAPD) markers. J. Amer. Soc. Hort. Sci123, 837–841.
159. Wu, G., Shott, B.J., Lawrence, E.B., Levine, E.B., Fitzsimmons, K.C., and Shah, D.M. (1995) Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in trangenic potato plants. Plant Cell 7, 1357–1368.PubMed
160. Yoshikawa, M., Tsuda, M., and Takeuchi, Y. (1993) Resistance to fungal diseases in transgenic tobacco plants expressing the phytoalexin elicitor-releasing factor, ###-1,3endoglucanase from soybean. Naturwissenschaften 80, 417–420.
161. Yoshikura, H. (1989) Suppression of focus formation by bovine papillomavirus-transformed cells by contact with non-transformed cells: Involvement of sugar(s) and phosphorylation. Intl. J. Cancer 44, 885–891.
162. Yoshikura, H. (1989) Suppression of focus formation by bovine papillomavirus-transformed cells by contact with non-transformed cells: Involvement of sugar(s) and phosphorylation. Intl. J Cancer 44, 885–891.
163. Zui-jun S. Shu-zhi, H.Kan-xi L., Liv, L.Ying G (1984) Breeding of olive trees for freesing resistance. In: Shan-an H., Hing (eds) Olive acclimatation and breeding. Najing PCR pp 429–445 (in Chinese).

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